Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/27517
Title: Low-Cost, Open-Source Device for High-Performance Fluorescence Detection of Isothermal Nucleic Acid Amplification Reactions.
Austin Authors: Buultjens, Andrew H;Vandelannoote, Koen;Sharkey, Liam K;Howden, Benjamin P ;Monk, Ian R;Lee, Jean Y H;Stinear, Timothy P
Affiliation: Department of Infectious Diseases, Monash Health, 246 Clayton Road, Clayton 3168, Victoria, Australia
Department of Microbiology and Immunology, The University of Melbourne at The Doherty Institute for Infection and Immunity, 792 Elizabeth Street, Melbourne 3000, Victoria, Australia
Microbiological Diagnostic Unit Public Health Laboratory, Level 1, The University of Melbourne at The Doherty Institute for Infection and Immunity, 792 Elizabeth Street, Melbourne 3000, Victoria, Australia
Doherty Applied Microbial Genomics, Department of Microbiology and Immunology, The University of Melbourne at The Doherty Institute for Infection and Immunity, 792 Elizabeth Street, Melbourne 3000, Victoria, Australia
Infectious Diseases
Issue Date: Oct-2021
Date: 2021-09-14
Publication information: ACS Biomaterials Science & Engineering 2021; 7(10): 4982-4990
Abstract: The ability to detect SARS-CoV-2 is critical to implementing evidence-based strategies to address the COVID-19 global pandemic. Expanding SARS-CoV-2 diagnostic ability beyond well-equipped laboratories widens the opportunity for surveillance and control efforts. However, such advances are predicated on the availability of rapid, scalable, accessible, yet high-performance diagnostic platforms. Methods to detect viral RNA using reverse transcription loop-mediated isothermal amplification (RT-LAMP) show promise as rapid and field-deployable tests; however, the per-unit costs of the required diagnostic hardware can be a barrier for scaled deployment. Here, we describe a diagnostic hardware configuration for LAMP technology, named the FABL-8, that can be built for approximately US$380 per machine and provide results in under 30 min. Benchmarking showed that FABL-8 has a similar performance to a high-end commercial instrument for detecting fluorescence-based LAMP reactions. Performance testing of the instrument with RNA extracted from a SARS-CoV-2 virus dilution series revealed an analytical detection sensitivity of 50 virus copies per microliter-a detection threshold suitable to detect patient viral load in the first few days following symptom onset. In addition to the detection of SARS-CoV-2, we show that the system can be used to detect the presence of two bacterial pathogens, demonstrating the versatility of the platform for the detection of other pathogens. This cost-effective and scalable hardware alternative allows democratization of the instrumentation required for high-performance molecular diagnostics, such that it could be available to laboratories anywhere-supporting infectious diseases surveillance and research activities in resource-limited settings.
URI: https://ahro.austin.org.au/austinjspui/handle/1/27517
DOI: 10.1021/acsbiomaterials.1c01105
ORCID: 0000-0002-5984-1328
0000-0003-0150-123X
Journal: ACS Biomaterials Science & Engineering
PubMed URL: 34521204
Type: Journal Article
Subjects: SARS-CoV-2 detection
decentralized diagnostics
loop-mediated isothermal amplification
low-cost molecular diagnostics
point of care diagnostics
rapid diagnostic test
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