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Title: Cell-Extrinsic Differentiation Block Mediated by EphA3 in Pre-Leukaemic Thymus Contributes to Disease Progression.
Austin Authors: Pliego Zamora, Adriana C;Ranasinghe, Hansini;Lisle, Jessica E;Ng, Chun Ki;Huang, Stephen;Wadlow, Racheal;Scott, Andrew M ;Boyd, Andrew W;Slape, Christopher I
Affiliation: Department of Medicine, The University of Queensland, Brisbane 4072, Australia
Faculty of Medicine, University of Melbourne, Melbourne 3000, Australia
The University of Queensland Diamantina Institute, The University of Queensland, Brisbane 4102, Australia
Olivia Newton-John Cancer Research Institute
La Trobe University, Heidelberg 3084, Australia
Issue Date: 31-Jul-2021 2021-07-31
Publication information: Cancers 2021; 13(15): 3858
Abstract: We recently characterised the NUP98-HOXD13 (NHD13) mouse as a model of T-cell pre-leukaemia, featuring thymocytes that can engraft in recipient animals and progress to T-cell acute lymphoblastic leukaemia (T-ALL). However, loss of this engraftment ability by deletion of Lyl1 did not result in any loss of leukemogenesis activity. In the present study, we observe that NHD13 thymocytes overexpress EPHA3, and we characterise thymocyte behaviour in NHD13 mice with deletion of EphA3, which show a markedly reduced incidence of T-ALL. Deletion of EphA3 from the NHD13 mice does not prevent the abnormal accumulation or transplantation ability of these thymocytes. However, upon transplantation, these cells are unable to block the normal progression of recipient wild type (WT) progenitor cells through the normal developmental pathway. This is in contrast to the EphA3+/+ NHD13 thymocytes, which block the progression of incoming WT progenitors past the DN1 stage. Therefore, EphA3 is not critical for classical self-renewal, but is essential for mediating an interaction between the abnormally self-renewing cells and healthy progenitors-an interaction that results in a failure of the healthy cells to differentiate normally. We speculate that this may orchestrate a loss of healthy cell competition, which in itself has been demonstrated to be oncogenic, and that this may explain the decrease in T-ALL incidence in the absence of EphA3. We suggest that pre-leukaemic self-renewal in this model is a complex interplay of cell-intrinsic and -extrinsic factors, and that multiple redundant pathways to leukaemogenesis are active.
DOI: 10.3390/cancers13153858
ORCID: 0000-0003-0581-740X
Journal: Cancers
PubMed URL: 34359759
ISSN: 2072-6694
Type: Journal Article
Subjects: Eph
cell competition
Appears in Collections:Journal articles

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