Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/26161
Title: EGFRvIII Promotes Cell Survival during Endoplasmic Reticulum Stress through a Reticulocalbin 1-Dependent Mechanism.
Austin Authors: Gomez, Juliana;Areeb, Zammam;Stuart, Sarah F;Nguyen, Hong P T;Paradiso, Lucia;Zulkifli, Ahmad;Madan, Sonakshi;Rajagopal, Vijay;Montgomery, Magdalene K;Gan, Hui K ;Scott, Andrew M ;Jones, Jordan;Kaye, Andrew H;Morokoff, Andrew P;Luwor, Rodney B
Affiliation: Department of Neurosurgery, Hadassah Hebrew University Medical Centre, Jerusalem 91120, Israel
Department of Surgery, The University of Melbourne, The Royal Melbourne Hospital, Parkville, VIC 3050, Australia
Department of Neurosurgery, The Royal Melbourne Hospital, Parkville, VIC 3050, Australia
Olivia Newton-John Cancer Research Institute
Department of Physiology, The University of Melbourne, Parkville, VIC 3010, Australia
Cell Structure and Mechanobiology Group, Department of Biomedical Engineering, Melbourne School of Engineering, The University of Melbourne, Parkville, VIC 3010, Australia
Issue Date: 10-Mar-2021
Date: 2021
Publication information: Cancers 2021; 13(6): 1198
Abstract: Reticulocalbin 1 (RCN1) is an endoplasmic reticulum (ER)-residing protein, involved in promoting cell survival during pathophysiological conditions that lead to ER stress. However, the key upstream receptor tyrosine kinase that regulates RCN1 expression and its potential role in cell survival in the glioblastoma setting have not been determined. Here, we demonstrate that RCN1 expression significantly correlates with poor glioblastoma patient survival. We also demonstrate that glioblastoma cells with expression of EGFRvIII receptor also have high RCN1 expression. Over-expression of wildtype EGFR also correlated with high RCN1 expression, suggesting that EGFR and EGFRvIII regulate RCN1 expression. Importantly, cells that expressed EGFRvIII and subsequently showed high RCN1 expression displayed greater cell viability under ER stress compared to EGFRvIII negative glioblastoma cells. Consistently, we also demonstrated that RCN1 knockdown reduced cell viability and exogenous introduction of RCN1 enhanced cell viability following induction of ER stress. Mechanistically, we demonstrate that the EGFRvIII-RCN1-driven increase in cell survival is due to the inactivation of the ER stress markers ATF4 and ATF6, maintained expression of the anti-apoptotic protein Bcl-2 and reduced activity of caspase 3/7. Our current findings identify that EGFRvIII regulates RCN1 expression and that this novel association promotes cell survival in glioblastoma cells during ER stress.
URI: https://ahro.austin.org.au/austinjspui/handle/1/26161
DOI: 10.3390/cancers13061198
ORCID: 0000-0003-0543-365X
0000-0002-5509-402X
0000-0001-7319-8546
0000-0002-3020-4245
Journal: Cancers
PubMed URL: 33801941
ISSN: 2072-6694
Type: Journal Article
Subjects: EGFRvIII
ER stress
RCN1
apoptosis
glioblastoma
Appears in Collections:Journal articles

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