Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/25956
Title: The calcitonin receptor regulates osteocyte lacunae acidity during lactation in mice.
Austin Authors: Davey, Rachel A;Clarke, Michele Verity;Golub, Suzanne;Russell, Patricia K;Zajac, Jeffrey D 
Affiliation: Medicine (University of Melbourne)
Issue Date: Apr-2021
Date: 2021-02-01
Publication information: The Journal of Endocrinology 2021; 249(1): 31-41
Abstract: The physiological role of calcitonin, and its receptor, the CTR, has long been debated. We previously provided the first evidence for a physiological role of the CTR to limit maternal bone loss during lactation in mice by a direct action on osteocytes to inhibit osteocytic osteolysis. We now extend these findings to show that CTR gene expression is upregulated 2-3 fold in whole bone of control mice at the end of pregnancy (E18) and lactation (P21) compared to virgin controls. This was associated with an increase in osteoclast activity evidenced by increases in osteoclast surface/bone surface and Dcstamp gene expression. To investigate the mechanism by which the CTR inhibits osteocytic osteolysis, in vivo acidification of the osteocyte lacunae during lactation (P14 days) was assessed using a pH indicator dye. A lower pH was observed in the osteocyte lacunae of lactating Global-CTRKOs compared to controls and was associated with an increase in the gene expression of ATPase H+ transporting V0 subunit D2 (Atp6v0d2) in whole bone of Global-CTRKOs at the end of lacation (P21). To determine whether the CTR is required for the replacement of mineral within the lacunae post-lactation, lacunar area was determined 3 weeks post-weaning. Comparison of the largest 20% of lacunae by area did not differ between Global-CTRKOs and controls post-lactation. These results provide evidence for CTR activation to inhibit osteocytic osteolysis during lactation being mediated by regulating the acidity of the lacunae microenvironment, whilst the CTR is dispensable for replacement of bone mineral within lacunae by osteocytes post-lactation.
URI: https://ahro.austin.org.au/austinjspui/handle/1/25956
DOI: 10.1530/JOE-20-0599
Journal: The Journal of Endocrinology
PubMed URL: 33638943
Type: Journal Article
Appears in Collections:Journal articles

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