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Title: | A Stabilizing Agent, PCA/DTPA, Improves Plasma Storage Life for the Chromsystems Vitamin C Assay up to Six Months. | Austin Authors: | Collie, Jake Tb;Hudson, Elizabeth P;Deane, Adam M;Bellomo, Rinaldo ;Greaves, Ronda F | Affiliation: | School of Health and Biomedical Sciences, RMIT University, Bundoora, Victoria, Australia Royal College of Pathologists Quality Assurance Programs Vitamins Advisory Committee, Sydney, New South Wales, Australia Dubbo Hospital, Western NSW Local Health District, Dubbo, New South Wales, Australia Department of Critical Care, The University of Melbourne, Melbourne, Australia Department of Intensive Care, Royal Melbourne Hospital, Parkville, Victoria, Australia Intensive Care School of Health and Biomedical Sciences, RMIT University, Bundoora, Victoria, Australia Royal College of Pathologists Quality Assurance Programs Vitamins Advisory Committee, Sydney, New South Wales, Australia Victorian Clinical Genetics Services, Murdoch Children's Research Institute, Parkville, Victoria, Australia Department of Paediatrics, University of Melbourne, Melbourne, VIC, Australia |
Issue Date: | 1-Jul-2021 | Publication information: | Annals of Laboratory Medicine 2021; 41(4): 414-418 | Abstract: | The commonly used Chromsystems vitamin C (ascorbate) assay (Munich, Germany) has a sample storage life of five days at -20°C. Stabilizing agents have been successfully used to increase longevity; however, their suitability with this commercial assay is unclear. We investigated the compatibility of a stabilizing agent, perchloric acid/diethylenetriaminepentaacetic acid (PCA/DTPA), with the Chromsystems assay. Plasma was stored at -80°C, with or without PCA/DTPA. Storage up to six months was assessed through baseline and repeat analyses, stability was assessed by comparing paired non-stabilized and PCA/DTPA-stabilized plasma, and performance was assessed using allowable performance specifications of an external quality assurance program. Ascorbate concentration was significantly lower in non-stabilized plasma than in paired PCA/DTPA-stabilized plasma, with a proportional difference of 11% (P=0.01). All storage analysis results were within the allowable performance specifications. Storage at -80°C prevented plasma ascorbate oxidation; however, substantial oxidation occurred during sample processing. In conclusion, PCA/DTPA significantly reduces ascorbate oxidation, and PCA/DTPA-stabilized ascorbate plasma is compatible with the Chromsystems assay and stable for up to six months, when stored at -80°C. | URI: | https://ahro.austin.org.au/austinjspui/handle/1/25791 | DOI: | 10.3343/alm.2021.41.4.414 | ORCID: | 0000-0002-4722-6395 0000-0001-7134-1556 0000-0002-7620-5577 0000-0002-1650-8939 0000-0001-7823-8797 |
Journal: | Annals of Laboratory Medicine | PubMed URL: | 33536361 | Type: | Journal Article | Subjects: | Ascorbate Chromsystems PCA/DTPA Stability Storage Vitamin C |
Appears in Collections: | Journal articles |
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