Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/25791
Title: A Stabilizing Agent, PCA/DTPA, Improves Plasma Storage Life for the Chromsystems Vitamin C Assay up to Six Months.
Austin Authors: Collie, Jake Tb;Hudson, Elizabeth P;Deane, Adam M;Bellomo, Rinaldo ;Greaves, Ronda F
Affiliation: School of Health and Biomedical Sciences, RMIT University, Bundoora, Victoria, Australia
Royal College of Pathologists Quality Assurance Programs Vitamins Advisory Committee, Sydney, New South Wales, Australia
Dubbo Hospital, Western NSW Local Health District, Dubbo, New South Wales, Australia
Department of Critical Care, The University of Melbourne, Melbourne, Australia
Department of Intensive Care, Royal Melbourne Hospital, Parkville, Victoria, Australia
Intensive Care
School of Health and Biomedical Sciences, RMIT University, Bundoora, Victoria, Australia
Royal College of Pathologists Quality Assurance Programs Vitamins Advisory Committee, Sydney, New South Wales, Australia
Victorian Clinical Genetics Services, Murdoch Children's Research Institute, Parkville, Victoria, Australia
Department of Paediatrics, University of Melbourne, Melbourne, VIC, Australia
Issue Date: 1-Jul-2021
Publication information: Annals of Laboratory Medicine 2021; 41(4): 414-418
Abstract: The commonly used Chromsystems vitamin C (ascorbate) assay (Munich, Germany) has a sample storage life of five days at -20°C. Stabilizing agents have been successfully used to increase longevity; however, their suitability with this commercial assay is unclear. We investigated the compatibility of a stabilizing agent, perchloric acid/diethylenetriaminepentaacetic acid (PCA/DTPA), with the Chromsystems assay. Plasma was stored at -80°C, with or without PCA/DTPA. Storage up to six months was assessed through baseline and repeat analyses, stability was assessed by comparing paired non-stabilized and PCA/DTPA-stabilized plasma, and performance was assessed using allowable performance specifications of an external quality assurance program. Ascorbate concentration was significantly lower in non-stabilized plasma than in paired PCA/DTPA-stabilized plasma, with a proportional difference of 11% (P=0.01). All storage analysis results were within the allowable performance specifications. Storage at -80°C prevented plasma ascorbate oxidation; however, substantial oxidation occurred during sample processing. In conclusion, PCA/DTPA significantly reduces ascorbate oxidation, and PCA/DTPA-stabilized ascorbate plasma is compatible with the Chromsystems assay and stable for up to six months, when stored at -80°C.
URI: https://ahro.austin.org.au/austinjspui/handle/1/25791
DOI: 10.3343/alm.2021.41.4.414
ORCID: 0000-0002-4722-6395
0000-0001-7134-1556
0000-0002-7620-5577
0000-0002-1650-8939
0000-0001-7823-8797
PubMed URL: 33536361
Type: Journal Article
Subjects: Ascorbate
Chromsystems
PCA/DTPA
Stability
Storage
Vitamin C
Appears in Collections:Journal articles

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