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Title: Peripheral neutrophil phenotypes during management of periodontitis.
Austin Authors: Medara, Nidhi;Lenzo, Jason C;Walsh, Katrina A;Reynolds, Eric C;O'Brien-Simpson, Neil M;Darby, Ivan B
Affiliation: Centre for Oral Health Research, Melbourne Dental School, The University of Melbourne, Carlton, VIC, Australia
Melbourne Dental School, The University of Melbourne, Carlton, VIC, Australia
Austin Health
Issue Date: Jan-2021
Date: 2020-08-17
Publication information: Journal of Periodontal Research 2020; 56(1): 58-68
Abstract: Neutrophils are emerging as a key player in periodontal pathogenesis. The surface expression of cellular markers enables functional phenotyping of neutrophils which have distinct roles in disease states. This study aimed to evaluate the effect of periodontal management on neutrophil phenotypes in peripheral blood in periodontitis patients over one year. Peripheral blood and the periodontal parameters, mean probing depth and percentage of sites with bleeding on probing (%BOP), were collected from 40 healthy controls and 54 periodontitis patients at baseline and 3-, 6- and 12-¬†months post-treatment. Flow cytometry was used to identify CD11b+ , CD16b+ , CD62L- and CD66b+ expression on neutrophils, neutrophil maturation stages as promyelocytes (CD11b- CD16b- ), metamyelocytes (CD11b+ CD16b- ) and mature neutrophils (CD11b+ CD16b+ ), and suppressive neutrophil phenotype as bands (CD16dim CD62Lbright ), normal neutrophils (CD16bright CD62Lbright ) and suppressive neutrophils (CD16bright CD62Ldim ). CD62L- expression decreased with treatment. No differences were observed in neutrophil maturation stages in health or disease upon treatment. Suppressive and normal neutrophils showed a reciprocal relationship, where suppressive neutrophils decreased with treatment and normal neutrophils increased with treatment. In addition, %BOP was associated with suppressive neutrophils. This study demonstrates that management of periodontitis significantly modifies distinct neutrophil phenotypes in peripheral blood. Suppressive neutrophils may play a role in the pathogenesis of periodontitis. However, their exact role is unclear and requires further investigation.
DOI: 10.1111/jre.12793
ORCID: 0000-0002-5450-0647
Journal: Journal of Periodontal Research
PubMed URL: 32803891
Type: Journal Article
Subjects: granulocyte precursor cells
periodontal debridement
suppressive neutrophils
Appears in Collections:Journal articles

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