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Title: Quantitation and chemical coding of enteroendocrine cell populations in the human jejunum.
Austin Authors: Fazio Coles, Therese E;Fothergill, Linda J;Hunne, Billie;Nikfarjam, Mehrdad ;Testro, Adam;Callaghan, Brid;McQuade, Rachel M;Furness, John B
Affiliation: Department of Surgery, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia
Department of Anatomy & Neuroscience, University of Melbourne, Parkville, Victoria, 3010, Australia
Florey Institute of Neuroscience and Mental Health, Parkville, Victoria, 3010, Australia
Victorian Liver Transplant Unit, Austin Health, Heidelberg, Victoria, Australia
Issue Date: Jan-2020
Date: 2019-09-02
Publication information: Cell and tissue research 2020; 379(1): 109-120
Abstract: Recent studies reveal substantial species and regional differences in enteroendocrine cell (EEC) populations, including differences in patterns of hormone coexpression, which limit extrapolation between animal models and human. In this study, jejunal samples, with no histologically identifiable pathology, from patients undergoing Whipple's procedure were investigated for the presence of gastrointestinal hormones using double- and triple-labelling immunohistochemistry and high-resolution confocal microscopy. Ten hormones (5-HT, CCK, secretin, proglucagon-derived peptides, PYY, GIP, somatostatin, neurotensin, ghrelin and motilin) were localised in EEC of the human jejunum. If only single staining is considered, the most numerous EEC were those containing 5-HT, CCK, ghrelin, GIP, motilin, secretin and proglucagon-derived peptides. All hormones had some degree of colocalisation with other hormones. This included a population of EEC in which GIP, CCK and proglucagon-derived peptides are costored, and four 5-HT cell populations, 5-HT/GIP, 5-HT/ghrelin, 5-HT/PYY, and 5-HT/secretin cell groups, and a high degree of overlap between motilin and ghrelin. The presence of 5-HT in many secretin cells is consistent across species, whereas lack of 5-HT and CCK colocalisation distinguishes human from mouse. It seems likely that the different subclasses of 5-HT cells subserve different roles. At a subcellular level, we examined the vesicular localisation of secretin and 5-HT, and found these to be separately stored. We conclude that hormone-containing cells in the human jejunum do not comply with a one-cell, one-hormone classification and that colocalisations of hormones are likely to define subtypes of EEC that have different roles.
DOI: 10.1007/s00441-019-03099-3
ORCID: 0000-0002-0219-3438
Journal: Cell and tissue research
PubMed URL: 31478137
Type: Journal Article
Subjects: 5-Hydroxytryptamine
Enteroendocrine cells
Gastrointestinal hormones
Glucose-dependent insulinotropic peptide
Appears in Collections:Journal articles

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