Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder.

2019-08-01

Bozaoglu, Kiymet

Gao, Yujing

Stanley, Edouard

Fanjul-Fernández, Miriam

Brown, Natasha J

Pope, Kate

Green, Cherie C

Vlahos, Katerina

Sourris, Koula

Bahlo, Melanie

Delatycki, Martin

Scheffer, Ingrid E

Lockhart, Paul J

Journal Article

0000-0002-3160-2106

0000-0002-2311-2174

10.1016/j.scr.2019.101516

We have generated and characterized seven human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) from a single family, including unaffected and affected individuals clinically diagnosed with Autism Spectrum Disorder (ASD). The reprogramming of the PBMCs was performed using non-integrative Sendai virus containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. All iPSC lines exhibited a normal karyotype and pluripotency was validated by immunofluorescence, flow cytometry and their ability to differentiate into the three embryonic germ layers. These iPSC lines are a valuable resource to study the molecular mechanisms underlying ASD.

link

Stem cell research 2019-08-01; 39: 101516

Stem cell research