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Title: Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder.
Austin Authors: Bozaoglu, Kiymet;Gao, Yujing;Stanley, Edouard;Fanjul-Fernández, Miriam;Brown, Natasha J;Pope, Kate;Green, Cherie C;Vlahos, Katerina;Sourris, Koula;Bahlo, Melanie;Delatycki, Martin;Scheffer, Ingrid E ;Lockhart, Paul J
Affiliation: Department of Neurology, Royal Children's Hospital, Melbourne, Australia
Florey Institute, Melbourne, Victoria, Australia
Murdoch Children's Research Institute, Parkville, Australia
Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia
Victorian Clinical Genetics Services, Victoria, Australia
Department of Paediatrics, University of Melbourne, Parkville, Australia
Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australia
Olga Tennison Autism Research Centre, School of Psychology and Public Health, La Trobe University, Bundoora, Victoria, Australia
Department of Medicine, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Department of Medical Biology, University of Melbourne, Melbourne, Australia
Issue Date: 1-Aug-2019 2019-08-01
Publication information: Stem cell research 2019-08-01; 39: 101516
Abstract: We have generated and characterized seven human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) from a single family, including unaffected and affected individuals clinically diagnosed with Autism Spectrum Disorder (ASD). The reprogramming of the PBMCs was performed using non-integrative Sendai virus containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. All iPSC lines exhibited a normal karyotype and pluripotency was validated by immunofluorescence, flow cytometry and their ability to differentiate into the three embryonic germ layers. These iPSC lines are a valuable resource to study the molecular mechanisms underlying ASD.
DOI: 10.1016/j.scr.2019.101516
ORCID: 0000-0002-3160-2106
PubMed URL: 31415975
Type: Journal Article
Appears in Collections:Journal articles

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