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Title: Immunoprofiling of Breast Cancer Antigens Using Antibodies Derived from Local Lymph Nodes.
Austin Authors: Young, Anna Rachel;Da Gama Duarte, Jessica;Coulson, Rhiannon;O'Brien, Megan;Deb, Siddhartha;Lopata, Alex;Behren, Andreas;Mathivanan, Suresh;Lim, Elgene;Meeusen, Els
Affiliation: CancerProbe Pty Ltd, PO Box 2237, Prahran 3181, Australia
Garvan Institute of Medical Research, St Vincent's Clinical School, Darlinghurst, NSW 2010, Australia
Consultant Pathologist, Anatpath. 120 Gardenvale Rd, Gardenvale Melbourne 3185, Australia
La Trobe Institute for Molecular Science (LIMS), La Trobe University, Melbourne 3086, Australia
Olivia Newton-John Cancer Research Institute, Heidelberg, Victoria, Australia
School of Cancer Medicine, La Trobe University, Melbourne 3086, Australia
Issue Date: 16-May-2019
Date: 2019-05-16
Publication information: Cancers 2019; 11(5): E682
Abstract: Tumor antigens are responsible for initiating an immune response in cancer patients, and their identification may provide new biomarkers for cancer diagnosis and targets for immunotherapy. The general use of serum antibodies to identify tumor antigens has several drawbacks, including dilution, complex formation, and background reactivity. In this study, antibodies were generated from antibody-secreting cells (ASC) present in tumor-draining lymph nodes of 20 breast cancer patients (ASC-probes) and were used to screen breast cancer cell lines and protein microarrays. Half of the ASC-probes reacted strongly against extracts of the MCF-7 breast cancer cell line, but each with a distinct antigen recognition profile. Three of the positive ASC-probes reacted differentially with recombinant antigens on a microarray containing cancer-related proteins. The results of this study show that lymph node-derived ASC-probes provide a highly specific source of tumor-specific antibodies. Each breast cancer patient reacts with a different antibody profile which indicates that targeted immunotherapies may need to be personalized for individual patients. Focused microarrays in combination with ASC-probes may be useful in providing immune profiles and identifying tumor antigens of individual cancer patients.
DOI: 10.3390/cancers11050682
ORCID: 0000-0003-4289-5204
Journal: Cancers
PubMed URL: 31100936
ISSN: 2072-6694
Type: Journal Article
Subjects: antibody secreting cell
breast cancer
immune profile
lymph node
tumor antigen
Appears in Collections:Journal articles

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