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Title: A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma.
Austin Authors: Stringer, Brett W;Day, Bryan W;D'Souza, Rochelle C J;Jamieson, Paul R;Ensbey, Kathleen S;Bruce, Zara C;Lim, Yi Chieh;Goasdoué, Kate;Offenhäuser, Carolin;Akgül, Seçkin;Allan, Suzanne;Robertson, Thomas;Lucas, Peter;Tollesson, Gert;Campbell, Scott;Winter, Craig;Do, Hongdo;Dobrovic, Alexander ;Inglis, Po-Ling;Jeffree, Rosalind L;Johns, Terrance G;Boyd, Andrew W
Affiliation: QIMR Berghofer Medical Research Institute, Brisbane, Australia
Olivia Newton-John Cancer Wellness and Research Centre, Austin Health, Heidelberg, Victoria, Australia
The University of Queensland, Brisbane, Australia
Hudson Institute of Medical Research, Clayton, Victoria, Australia
Royal Brisbane and Women's Hospital, Brisbane, Australia
Issue Date: 20-Mar-2019
Date: 2019
Publication information: Scientific Reports 2019; 9(1): 4902
Abstract: Low-passage, serum-free cell lines cultured from patient tumour tissue are the gold-standard for preclinical studies and cellular investigations of glioblastoma (GBM) biology, yet entrenched, poorly-representative cell line models are still widely used, compromising the significance of much GBM research. We submit that greater adoption of these critical resources will be promoted by the provision of a suitably-sized, meaningfully-described reference collection along with appropriate tools for working with them. Consequently, we present a curated panel of 12 readily-usable, genetically-diverse, tumourigenic, patient-derived, low-passage, serum-free cell lines representing the spectrum of molecular subtypes of IDH-wildtype GBM along with their detailed phenotypic characterisation plus a bespoke set of lentiviral plasmids for bioluminescent/fluorescent labelling, gene expression and CRISPR/Cas9-mediated gene inactivation. The cell lines and all accompanying data are readily-accessible via a single website, Q-Cell ( and all plasmids are available from Addgene. These resources should prove valuable to investigators seeking readily-usable, well-characterised, clinically-relevant, gold-standard models of GBM.
DOI: 10.1038/s41598-019-41277-z
ORCID: 0000-0003-4552-9736
Journal: Scientific Reports
PubMed URL: 30894629
Type: Journal Article
Appears in Collections:Journal articles

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