Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/20212
Title: Reconstruction of the Genomes of Drug-Resistant Pathogens for Outbreak Investigation through Metagenomic Sequencing.
Austin Authors: Mu, Andre;Kwong, Jason C ;Isles, Nicole S;Gonçalves da Silva, Anders;Schultz, Mark B;Ballard, Susan A;Lane, Courtney R;Carter, Glen P;Williamson, Deborah A;Seemann, Torsten;Stinear, Timothy P;Howden, Benjamin P 
Affiliation: Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Victoria, Australia
Department of Infectious Diseases, Austin Health, Heidelberg, Victoria, Australia
Doherty Applied Microbial Genomics, Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Victoria, Australia
Melbourne Bioinformatics, University of Melbourne, Melbourne, Victoria, Australia
Issue Date: 16-Jan-2019
metadata.dc.date: 2019-01-16
Publication information: mSphere 2019; 4(1): e00529-19
Abstract: Culture-independent methods that target genome fragments have shown promise in identifying certain pathogens, but the holy grail of comprehensive pathogen genome detection from microbiologically complex samples for subsequent forensic analyses remains a challenge. In the context of an investigation of a nosocomial outbreak, we used shotgun metagenomic sequencing of a human fecal sample and a neural network algorithm based on tetranucleotide frequency profiling to reconstruct microbial genomes and tested the same approach using rectal swabs from a second patient. The approach rapidly and readily detected the genome of Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae in the patient fecal specimen and in the rectal swab sample, achieving a level of strain resolution that was sufficient for confident transmission inference during a highly clonal outbreak. The analysis also detected previously unrecognized colonization of the patient by vancomycin-resistant Enterococcus faecium, another multidrug-resistant bacterium.IMPORTANCE The study results reported here perfectly demonstrate the power and promise of clinical metagenomics to recover genome sequences of important drug-resistant bacteria and to rapidly provide rich data that inform outbreak investigations and treatment decisions, independently of the need to culture the organisms.
URI: http://ahro.austin.org.au/austinjspui/handle/1/20212
DOI: 10.1128/mSphere.00529-18
ORCID: 0000-0002-7689-6531
0000-0003-0237-1473
PubMed URL: 30651402
Type: Journal Article
Subjects: Klebsiella pneumoniae
antimicrobial resistance
metagenomics
microbiome
vancomycin-resistant enterococci
Appears in Collections:Journal articles

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