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Title: The Effects of Diabetes and High-Fat Diet on Polymodal Nociceptor and Cold Thermoreceptor Nerve Terminal Endings in the Corneal Epithelium.
Austin Authors: Alamri, Abdulhakeem S;Brock, James A;Herath, Chandana B;Rajapaksha, Indu G;Angus, Peter W ;Ivanusic, Jason J
Affiliation: Gastroenterology and Hepatology
Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne, Victoria, Australia
Medicine (University of Melbourne)
Issue Date: 2-Jan-2019
Publication information: Investigative Ophthalmology & Visual Science 2019; 60(1): 209-217
Abstract: There is a substantial body of evidence indicating that corneal sensory innervation is affected by pathology in a range of diseases. However, there are no published studies that have directly assessed whether the nerve fiber density of the different subpopulations of corneal sensory neurons are differentially affected. The present study explored the possibility that the intraepithelial nerve fiber density of corneal polymodal nociceptors and cold thermoreceptors are differentially affected in mice fed with a high-fat high cholesterol (HFHC; 21% fat, 2% cholesterol) diet and in those that also have diabetes. The mice were fed the HFHC diet for the duration of the experiment (up to 40 weeks). Mice in the diabetes group had hyperglycaemia induced with streptozotocin after 15 weeks on the HFHC diet. Age-matched control animals were fed a standard diet. All corneal nerve fibers were labeled with a pan neuronal antibody (antiprotein gene product 9.5), and polymodal nociceptors and cold thermoreceptors were labeled with antibodies directed against transient receptor potential cation channel, subfamily V, member 1 and transient receptor potential cation channel subfamily M member 8, respectively. The mice fed a HFHC diet and those that in addition have hyperglycemia have similar reductions in corneal nerve fiber density consistent with small fiber neuropathy. Importantly, both treatments more markedly affected the intraepithelial axons of cold thermoreceptors than those of polymodal nociceptors. The results provide evidence that distinct subpopulations of corneal sensory neurons can be differentially affected by pathology.
DOI: 10.1167/iovs.18-25788
Journal: Investigative Ophthalmology & Visual Science
PubMed URL: 30641549
Type: Journal Article
Appears in Collections:Journal articles

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