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Title: Regulation through microRNAs in Response to Low-Energy N+ Ion Irradiation in Oryza sativa.
Austin Authors: Li, Yalin;Wang, Weidong;Wang, Tao;Wouters, Merridee A;Yin, Yue;Jiao, Zhen;Ma, Lixia;Zhang, Fengqiu
Affiliation: Henan Key Laboratory of Ion-beam Bioengineering, Zhengzhou University, Zhengzhou 450000, China
School of Nursing, Zhengzhou University, Zhengzhou 450000, China
Olivia Newton-John Cancer Research Institute, Heidelberg, Victoria, Australia
School of Cancer Medicine, La Trobe University, Bundoora 3086, Australia
Zhengzhou Research Base State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450000, China
School of Statistics, Henan University of Finance and Economics, Zhengzhou 450000, China
Issue Date: 30-Nov-2018 2018-11-30
Publication information: Radiation research 2019; 191(2): 189-200
Abstract: MicroRNAs (miRNAs) are a non-coding regulatory RNAs that play significant roles in plant growth and development, especially in the stress response. Low-energy ion radiation, a type of environmental stress, can cause multiple biological effects. To understand the roles of miRNAs in response to low-energy N+ ion radiation in Oryza sativa, high-throughput sequencing of small RNAs was carried out to detect the expression of miRNAs in the shoots of the rice after 2 × 1017 N+/cm2 irradiation. The differentially expressed 28 known miRNAs were identified, 17 of these identified miRNAs were validated by real-time quantitative fluorescent PCR (q-PCR), including 9 up-regulated miRNAs (miR1320-3p, miR1320-5p, miR156b-3p, miR156c-5p, miR156c-3p/g-3p, miR156l-5p, miR398b and miR6250) and 8 down-regulated miRNAs (miR156a/e/i, miR156k, miR160f-5p, miR166j-5p, miR1846e and miR399d). In addition, 45 novel radiation-responsive miRNAs were predicted, and 8 of them were verified by q-PCR. The target genes of radiation-responsive miRNAs were predicted and gene function enrichment analysis was performed with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The expression of 9 targets of 4 known miRNA families (miR156, miR399, miR1320 and miR398) and 2 targets of 2 novel miRNAs were quantified by q-PCR, and a strong negative regulation relation between miRNAs and their targets were observed. Those targets including SQUAMOSA promoter-binding-like protein (SPL) genes, copper/zinc superoxide dismutase (Cu/Zn-SOD), copper chaperone for SOD (CCS1) and electron transporter/ heat-shock protein binding protein (HSP), which are involved in growth and defense against various stresses, especially associated with reactive oxygen species (ROS) scavenging. This work provides important information for understanding the ROS generation and elimination mechanisms closely related to miRNAs in rice seedlings after low-energy N+ radiation exposure.
DOI: 10.1667/RR15125.1
ORCID: 0000-0002-2121-912X
PubMed URL: 30499385
Type: Journal Article
Appears in Collections:Journal articles

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