Please use this identifier to cite or link to this item:
Title: Distribution and characterisation of CCK containing enteroendocrine cells of the mouse small and large intestine.
Austin Authors: Fakhry, Josiane;Wang, Joyce;Martins, Patricia;Fothergill, Linda J;Hunne, Billie;Prieur, Pierre;Shulkes, Arthur;Rehfeld, Jens F;Callaghan, Brid;Furness, John B
Affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia
Department of Surgery, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia
Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
Florey Institute of Neuroscience and Mental Health, Parkville, VIC, 3010, Australia
Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
Issue Date: Aug-2017 2017-04-17
Publication information: Cell and tissue research 2017; 369(2): 245-253
Abstract: There is general consensus that enteroendocrine cells, EEC, containing the enteric hormone cholecystokinin (CCK) are confined to the small intestine and predominate in the duodenum and jejunum. Contrary to this, EEC that express the gene for CCK have been isolated from the large intestine of the mouse and there is evidence for EEC that contain CCK-like immunoreactivity in the mouse colon. However, the human and rat colons do not contain CCK cells. In the current study, we use immunohistochemistry to investigate CCK peptide presence in endocrine cells, PCR to identify cck transcripts and chromatography to identify CCK peptide forms in the mouse small and large intestine. The colocalisation of CCK and 5-HT, hormones that have been hypothesised to derive from cells of different lineages, was also investigated. CCK immunoreactivity was found in EEC throughout the mouse small and large intestine but positive cells were rare in the rectum. Immunoreactive EEC were as common in the caecum and proximal colon as they were in the duodenum and jejunum. CCK gene transcripts were found in the mucosa throughout the intestine but mRNA for gastrin, a hormone that can bind some anti-CCK antibodies, was only found in the stomach and duodenum. Characterisation of CCK peptides of the colon by extraction, chromatographic separation and radioimmunoassay revealed bioactive amidated and sulphated forms, including CCK-8 and CCK-33. Moreover, CCK-containing EEC in the large intestine bound antibodies that target the biologically active sulfated form. Colocalisation of CCK and 5-HT occurred in a proportion of EEC throughout the small intestine and in the caecum but these hormones were not colocalised in the colon, where there was CCK and PYY colocalisation. It is concluded that authentic, biologically active, CCK occurs in EEC of the mouse large intestine.
DOI: 10.1007/s00441-017-2612-1
PubMed URL: 28413860
Type: Journal Article
Subjects: 5-hydroxytryptamine
Gastrointestinal hormones
Peptide YY
Appears in Collections:Journal articles

Show full item record

Google ScholarTM


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.