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Title: Plasmacytoid dendritic cell heterogeneity is defined by CXCL10 expression following TLR7 stimulation.
Austin Authors: Marsman, Casper;Lafouresse, Fanny;Liao, Yang;Baldwin, Tracey M;Mielke, Lisa A;Hu, Yifang;Mack, Matthias;Hertzog, Paul J;de Graaf, Carolyn A;Shi, Wei;Groom, Joanna R
Affiliation: Division of Molecular Medicine, Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia
Divisions of Immunology and Molecular Immunology, Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia
Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia
Division of Bioinformatics, Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia
Olivia Newton-John Cancer Research Institute, Heidelberg, Victoria, Australia
Department of Internal Medicíne/Nephrology, University Hospital Regensburg, Franz-Josef-Strauss Allee 11, 93042, Regensburg, Germany
Hudson Institute of Medical Research, Clayton, VIC, 3168, Australia
Department of Computing and Information Systems, University of Melbourne, Parkville, VIC, Australia
La Trobe University School of Cancer Medicine, Heidelberg, VIC, 3084, Australia
Issue Date: 5-Jun-2018
Date: 2018-06-05
Publication information: Immunology and cell biology 2018; 96(10): 1083-1094
Abstract: Plasmacytoid dendritic cells (pDCs) play a critical role in bridging the innate and adaptive immune systems. pDCs are specialized type I interferon (IFN) producers, which has implicated them as initiators of autoimmune pathogenesis. However, little is known about the downstream effectors of type I IFN signaling that amplify autoimmune responses. Here, we have used a chemokine reporter mouse to determine the CXCR3 ligand responses in DCs subsets. Following TLR7 stimulation, conventional type 1 and type 2 DCs (cDC1 and cDC2, respectively) uniformly upregulate CXCL10. By contrast, the proportion of chemokine positive pDCs was significantly less, and stable CXCL10+ and CXCL10- populations could be distinguished. CXCL9 expression was induced in all cDC1s, in half of the cDC2 but not by pDCs. The requirement for IFNAR signaling for chemokine reporter expression was interrogated by receptor blocking and deficiency and shown to be critical for CXCR3 ligand expression in Flt3-ligand-derived DCs. Chemokine-producing potential was not concordant with the previously identified markers of pDC heterogeneity. Finally, we show that CXCL10+ and CXCL10- populations are transcriptionally distinct, expressing unique transcriptional regulators, IFN signaling molecules, chemokines, cytokines, and cell surface markers. This work highlights CXCL10 as a downstream effector of type I IFN signaling and suggests a division of labor in pDCs subtypes that likely impacts their function as effectors of viral responses and as drivers of inflammation.
DOI: 10.1111/imcb.12173
ORCID: 0000-0001-5251-7835
Journal: Immunology and cell biology
PubMed URL: 29870118
Type: Journal Article
Subjects: CXCR3
conventional DC
plasmacytoid DC
type 1 IFN
Appears in Collections:Journal articles

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