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dc.contributor.authorMarsman, Casper-
dc.contributor.authorLafouresse, Fanny-
dc.contributor.authorLiao, Yang-
dc.contributor.authorBaldwin, Tracey M-
dc.contributor.authorMielke, Lisa A-
dc.contributor.authorHu, Yifang-
dc.contributor.authorMack, Matthias-
dc.contributor.authorHertzog, Paul J-
dc.contributor.authorde Graaf, Carolyn A-
dc.contributor.authorShi, Wei-
dc.contributor.authorGroom, Joanna R-
dc.identifier.citationImmunology and cell biology 2018; 96(10): 1083-1094-
dc.description.abstractPlasmacytoid dendritic cells (pDCs) play a critical role in bridging the innate and adaptive immune systems. pDCs are specialized type I interferon (IFN) producers, which has implicated them as initiators of autoimmune pathogenesis. However, little is known about the downstream effectors of type I IFN signaling that amplify autoimmune responses. Here, we have used a chemokine reporter mouse to determine the CXCR3 ligand responses in DCs subsets. Following TLR7 stimulation, conventional type 1 and type 2 DCs (cDC1 and cDC2, respectively) uniformly upregulate CXCL10. By contrast, the proportion of chemokine positive pDCs was significantly less, and stable CXCL10+ and CXCL10- populations could be distinguished. CXCL9 expression was induced in all cDC1s, in half of the cDC2 but not by pDCs. The requirement for IFNAR signaling for chemokine reporter expression was interrogated by receptor blocking and deficiency and shown to be critical for CXCR3 ligand expression in Flt3-ligand-derived DCs. Chemokine-producing potential was not concordant with the previously identified markers of pDC heterogeneity. Finally, we show that CXCL10+ and CXCL10- populations are transcriptionally distinct, expressing unique transcriptional regulators, IFN signaling molecules, chemokines, cytokines, and cell surface markers. This work highlights CXCL10 as a downstream effector of type I IFN signaling and suggests a division of labor in pDCs subtypes that likely impacts their function as effectors of viral responses and as drivers of inflammation.-
dc.subjectconventional DC-
dc.subjectplasmacytoid DC-
dc.subjecttype 1 IFN-
dc.titlePlasmacytoid dendritic cell heterogeneity is defined by CXCL10 expression following TLR7 stimulation.-
dc.typeJournal Article-
dc.identifier.journaltitleImmunology and cell biology-
dc.identifier.affiliationDivision of Molecular Medicine, Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australiaen
dc.identifier.affiliationDivisions of Immunology and Molecular Immunology, Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia-
dc.identifier.affiliationDepartment of Medical Biology, University of Melbourne, Parkville, VIC, Australia-
dc.identifier.affiliationDivision of Bioinformatics, Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia-
dc.identifier.affiliationOlivia Newton-John Cancer Research Institute, Heidelberg, Victoria, Australia-
dc.identifier.affiliationDepartment of Internal Medicíne/Nephrology, University Hospital Regensburg, Franz-Josef-Strauss Allee 11, 93042, Regensburg, Germany-
dc.identifier.affiliationHudson Institute of Medical Research, Clayton, VIC, 3168, Australia-
dc.identifier.affiliationDepartment of Computing and Information Systems, University of Melbourne, Parkville, VIC, Australia-
dc.identifier.affiliationLa Trobe University School of Cancer Medicine, Heidelberg, VIC, 3084, Australia-
dc.type.austinJournal Article-
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
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