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Title: Cell Line Models of Molecular Subtypes of Colorectal Cancer.
Austin Authors: Mooi, Jennifer K ;Luk, Ian Y;Mariadason, John M 
Affiliation: Olivia Newton-John Cancer Research Institute, Heidelberg, Victoria, Australia
Issue Date: 2018
Publication information: Methods in molecular biology (Clifton, N.J.) 2018; 1765: 3-26
Abstract: Colorectal cancer (CRC) is a genetically diverse disease necessitating the need for well-characterized and reproducible models to enable its accurate investigation. Recent genomic analyses have confirmed that CRC cell lines accurately retain the key genetic alterations and represent the major molecular subtypes of primary CRC, underscoring their value as powerful preclinical models. In this chapter we detail the important issues to consider when using CRC cell lines, the techniques used for their appropriate molecular classification, and the methods by which they are cultured in vitro and as subcutaneous xenografts in immune-compromised mice. A panel of commonly available CRC cell lines that have been characterized for key molecular subtypes is also provided as a resource for investigators to select appropriate models to address specific research questions.
DOI: 10.1007/978-1-4939-7765-9_1
Journal: Methods in molecular biology (Clifton, N.J.)
PubMed URL: 29589298
Type: Journal Article
Subjects: Chromosomal instability
Colorectal cancer cells
CpG island methylator phenotype
DNA profiling
Microsatellite instability
Molecular subtype
Signaling pathways
Appears in Collections:Journal articles

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