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Title: Vitamin B1 in critically ill patients: needs and challenges
Austin Authors: Collie, Jake TB;Greaves, Ronda F;Jones, Oliver A H;Lam, Que;Eastwood, Glenn M ;Bellomo, Rinaldo 
Affiliation: School of Health and Biomedical Sciences, RMIT University, Bundoora, Australia
Dorevitch Pathology, Special Chemistry, Heidelberg, Victoria, Australia
Murdoch Children’s Research Institute, Parkville, Victoria, Australia
Australasian Association of Clinical Biochemists Vitamins Working Party
Australian Centre for Research on Separation Science, School of Science, RMIT University, Melbourne, Australia
Department of Pathology, Austin Health, Heidelberg, Victoria, Australia
Department of Intensive Care, Austin Health, Heidelberg, Victoria, Australia
School of Medicine, The University of Melbourne, Parkville, Australia
Issue Date: 22-Apr-2017 2017-04-22
Publication information: Clinical Chemistry and Laboratory Medicine 2017; online first: 22 April
Abstract: Background: Thiamine has a crucial role in energy production, and consequently thiamine deficiency (TD) has been associated with cardiac failure, neurological disorders, oxidative stress (lactic acidosis and sepsis) and refeeding syndrome (RFS). This review aims to explore analytical methodologies of thiamine compound quantification and highlight similarities, variances and limitations of current techniques and how they may be relevant to patients. Content: An electronic search of Medline, PubMed and Embase databases for original articles published in peer-reviewed journals was conducted. MethodsNow was used to search for published analytical methods of thiamine compounds. Keywords for all databases included “thiamine and its phosphate esters”, “thiamine methodology” and terms related to critical illness. Enquiries were also made to six external quality assurance (EQA) programme organisations for the inclusion of thiamine measurement. Summary: A total of 777 published articles were identified; 122 were included in this review. The most common published method is HPLC with florescence detection. Two of the six EQA organisations include a thiamine measurement programme, both measuring only whole-blood thiamine pyrophosphate (TPP). No standard measurement procedure for thiamine compound quantification was identified. Outlook: Overall, there is an absence of standardisation in measurement methodologies for thiamine in clinical care. Consequently, multiple variations in method practises are prohibiting the comparison of study results as they are not traceable to any higher order reference. Traceability of certified reference materials and reference measurement procedures is needed to provide an anchor to create the link between studies and help bring consensus on the clinical importance of thiamine.
DOI: 10.1515/cclm-2017-0054
PubMed URL:
Type: Journal Article
Subjects: Chromatography
Critically ill
Mass spectrometry
Vitamin B1
Appears in Collections:Journal articles

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