Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/16618
Title: PD-L1 and tumor infiltrating lymphocytes as prognostic markers in resected NSCLC
Austin Authors: Ameratunga, Malaka;Asadi, Khashayar ;Lin, Xihui;Walkiewicz, Marzena;Murone, Carmel ;Knight, Simon;Mitchell, Paul L R ;Boutros, Paul;John, Thomas 
Affiliation: Department of Medical Oncology, Austin Health, Olivia Newton-John Cancer and Wellness Centre, Heidelberg, Victoria, Australia
Department of Pathology, Austin Health, Heidelberg, Victoria, Australia
Informatics & Biocomputing Program, Ontario Institute for Cancer Research, Toronto, Canada
The Olivia Newton-John Cancer Research Institute, Heidelberg, Victoria, Australia
Department of Thoracics, Austin Health, Heidelberg, Victoria, Australia
Department of Medical Biophysics, University of Toronto, Toronto, Canada
Department of Pharmacology & Toxicology, University of Toronto, Toronto, Canada
School of Cancer Medicine, La Trobe University, Victoria, Australia
University of Melbourne, Melbourne, Victoria, Australia
Issue Date: 22-Apr-2017
Date: 2017-04-22
Publication information: PLoS One 2017: 11(4): e0153954
Abstract: INTRODUCTION: Immune checkpoint inhibition has shifted treatment paradigms in non-small cell lung cancer (NSCLC). Conflicting results have been reported regarding the immune infiltrate and programmed death-ligand 1 (PD-L1) as a prognostic marker. We correlated the immune infiltrate and PD-L1 expression with clinicopathologic characteristics in a cohort of resected NSCLC. METHODS: A tissue microarray was constructed using triplicate cores from consecutive resected NSCLC. Immunohistochemistry was performed for CD8, FOXP3 and PD-L1. Strong PD-L1 expression was predefined as greater than 50% tumor cell positivity. Matched nodal samples were assessed for concordance of PD-L1 expression. RESULTS: Of 522 patients, 346 were node-negative (N0), 72 N1 and 109 N2; 265 were adenocarcinomas (AC), 182 squamous cell cancers (SCC) and 75 other. Strong PD-L1 expression was found in 24% cases. In the overall cohort, PD-L1 expression was not associated with survival. In patients with N2 disease, strong PD-L1 expression was associated with significantly improved disease-free (DFS) and overall survival (OS) in multivariate analysis (HR 0.49, 95%CI 0.36-0.94, p = 0.031; HR 0.46, 95%CI 0.26-0.80, p = 0.006). In this resected cohort only 5% harboured EGFR mutations, whereas 19% harboured KRAS and 23% other. KRAS mutated tumors were more likely to highly express PD-L1 compared to EGFR (22% vs 3%). A stromal CD8 infiltrate was associated with significantly improved DFS in SCC (HR 0.70, 95%CI 0.50-0.97, p = 0.034), but not AC, whereas FOXP3 was not prognostic. Matched nodal specimens (N = 53) were highly concordant for PD-L1 expression (89%). CONCLUSION: PD-L1 expression was not prognostic in the overall cohort. PD-L1 expression in primary tumor and matched nodal specimens were highly concordant. The observed survival benefit in N2 disease requires confirmation.
URI: https://ahro.austin.org.au/austinjspui/handle/1/16618
DOI: 10.1371/journal.pone.0153954
Journal: PLoS One
PubMed URL: https://pubmed.ncbi.nlm.nih.gov/27104612
Type: Journal Article
Subjects: Lung neoplasms
Lymphocytes, tumor-infiltrating
Biomarkers, tumor
Antigens, CD274
Appears in Collections:Journal articles

Show full item record

Page view(s)

38
checked on Nov 22, 2024

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.