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Title: Muscle-specific androgen receptor deletion shows limited actions in myoblasts but not in myofibers in different muscles in vivo
Austin Authors: Rana, Kesha;Chiu, Maria WS;Russell, Patricia K;Skinner, Jarrod P;Lee, Nicole KL;Fam, Barbara C;Zajac, Jeffrey D ;MacLean, Helen E
Affiliation: Department of Medicine, University of Melbourne, Austin Health, Heidelberg, Victoria, Australia
Issue Date: 1-Aug-2016 2016-07-08
Publication information: Journal of Molecular Endocrinology 2016; 57(2): 125-138
Abstract: The aim of this study was to investigate the direct muscle cell-mediated actions of androgens by comparing two different mouse lines. The cre-loxP system was used to delete the DNA-binding activity of the androgen receptor (AR) in mature myofibers (MCK mARΔZF2) in one model and the DNA-binding activity of the AR in both proliferating myoblasts and myofibers (α-actin mARΔZF2) in another model. We found that hind-limb muscle mass was normal in MCK mARΔZF2 mice and that relative mass of only some hind-limb muscles was reduced in α-actin mARΔZF2 mice. This suggests that myoblasts and myofibers are not the major cellular targets mediating the anabolic actions of androgens on male muscle during growth and development. Levator ani muscle mass was decreased in both mouse lines, demonstrating that there is a myofiber-specific effect in this unique androgen-dependent muscle. We found that the pattern of expression of genes including c-myc, Fzd4 and Igf2 is associated with androgen-dependent changes in muscle mass; therefore, these genes are likely to be mediators of anabolic actions of androgens. Further research is required to identify the major targets of androgen actions in muscle, which are likely to include indirect actions via other tissues.
DOI: 10.1530/JME-15-0320
PubMed URL:
Type: Journal Article
Subjects: Androgen receptor
Appears in Collections:Journal articles

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