Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/16227
Title: Large scale production of a mammalian cell derived quadrivalent hepatitis C virus like particle vaccine
Austin Authors: Earnest-Silveira, Linda;Christiansen, Dale;Herrmann, S;Ralph, SA;Das, S;Gowans, EJ;Torresi, Joseph 
Affiliation: Austin Health, Heidelberg, Victoria, Australia
Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia
Department of Surgery, Austin Health, the University of Melbourne, Heidelberg, Victoria, Australia
Department of Biochemistry and Molecular Biology, Bio21 Institute of Molecular Science and Biotechnology, The University of Melbourne, Parkville, Victoria, Australia
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka, India
The Basil Hetzel Institute and Queen Elizabeth Hospital, University of Adelaide, South Australia, Australia
Issue Date: Oct-2016
metadata.dc.date: 2016-06-29
Publication information: Journal of Virological Methods 2016; 236: 87-92
Abstract: A method for the large-scale production of a quadrivalent mammalian cell derived hepatitis C virus-like particles (HCV VLPs) is described. The HCV core E1 and E2 coding sequences of genotype 1a, 1b, 2a or 3a were co-expressed in Huh7 cell factories using a recombinant adenoviral expression system. The structural proteins self-assembled into VLPs that were purified from Huh7 cell lysates by iodixanol ultracentrifugation and Stirred cell ultrafiltration. Electron microscopy, revealed VLPs of the different genotypes that are morphologically similar. Our results show that it is possible to produce large quantities of individual HCV genotype VLPs with relative ease thus making this approach an alternative for the manufacture of a quadrivalent mammalian cell derived HCV VLP vaccine.
URI: http://ahro.austin.org.au/austinjspui/handle/1/16227
DOI: 10.1016/j.jviromet.2016.06.012
ORCID: 0000-0002-8212-0887
PubMed URL: https://pubmed.ncbi.nlm.nih.gov/27373602
Type: Journal Article
Subjects: Hepatitis C
Quadrivalent vaccine
Virus-like particles
Appears in Collections:Journal articles

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