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Title: Relative contributions of advanced glycation and nitric oxide synthase inhibition to aminoguanidine-mediated renoprotection in diabetic rats
Austin Authors: Soulis, T;Cooper, Mark E;Sastra, S;Thallas, Vicki;Panagiotopoulos, Sianna ;Bjerrum, O J;Jerums, George 
Affiliation: Department of Medicine, University of Melbourne, Austin and Repatriation Medical Centre (Repatriation Campus), West Heidelberg, Australia
Issue Date: 1-Oct-1997
Publication information: Diabetologia; 40(10): 1141-51
Abstract: Advanced glycation end products (AGEs) have previously been shown to be increased in the diabetic kidney. Aminoguanidine, an inhibitor of advanced glycation, has been shown to attenuate the development of AGEs as well as the progression of renal disease in experimental diabetes. However, the precise mechanisms through which aminoguanidine acts remain to be elucidated since it is also able to act as an inhibitor of nitric oxide synthase (NOS). This study has therefore compared the effects of aminoguanidine with the effects of two other inhibitors of NOS, L-NAME and methylguanidine, on the development of experimental diabetic nephropathy. Diabetic rats were randomised to receive no treatment, aminoguanidine (1 g/l in drinking water), L-NAME (5 mg/l in drinking water) or methylguanidine (1 g/l in drinking water). Diabetic rats had increased levels of albuminuria and urinary nitrite/nitrate excretion when compared to control rats. Renal AGEs measured by fluorescence as well as by a carboxymethyllysine reactive radioimmunoassay, were elevated in diabetic rats. No changes in inducible NOS (iNOS) protein expression were detected in experimental diabetes nor did aminoguanidine affect iNOS expression. Aminoguanidine did not affect blood glucose or HbA1c but it did prevent increases in albuminuria, urinary nitrites/nitrates and renal AGE levels as measured by fluorescence and radioimmunoassay. L-NAME and methylguanidine did not retard the development of albuminuria, nor did they prevent increases in renal AGE levels, as assessed by fluorescence. However, these treatments did prevent increases in AGEs, as measured by radioimmunoassay. This study indicates that the renoprotective effect of aminoguanidine in experimental diabetes cannot be reproduced by L-NAME or methylguanidine. It is likely that the effect of aminoguanidine is mediated predominantly by decreased AGE formation rather than via NOS inhibition. It also raises the possibility that inhibition of fluorescent AGE formation may be more renoprotective than inhibition of the formation of carboxymethyllysine-containing AGEs.
Gov't Doc #: 9349594
DOI: 10.1007/s001250050799
ORCID: 0000-0002-0845-0001
Journal: Diabetologia
Type: Journal Article
Subjects: Administration, Oral
Cohort Studies
Diabetes Mellitus, Experimental.immunology.pathology.urine
Enzyme Inhibitors.administration & dosage.pharmacology
Glycosylation End Products, Advanced.immunology.metabolism.urine
Guanidines.administration & dosage.pharmacology
Kidney Glomerulus.drug effects.immunology.metabolism.pathology
Methylguanidine.administration & dosage.pharmacology
NG-Nitroarginine Methyl Ester.administration & dosage.pharmacology
Nitric Oxide Synthase.antagonists & inhibitors
Random Allocation
Rats, Sprague-Dawley
Time Factors
Appears in Collections:Journal articles

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