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Title: Increased epidermal growth factor in experimental diabetes related kidney growth in rats.
Austin Authors: Gilbert, Richard E;Cox, Allison J;McNally, P G;Wu, L L;Dziadek, M;Cooper, Mark E;Jerums, George 
Affiliation: Department of Medicine, Austin and Repatriation Medical Centre, University of Melbourne, Victoria, Australia
Issue Date: 1-Jul-1997
Publication information: Diabetologia; 40(7): 778-85
Abstract: Renal enlargement is a characteristic feature of human and experimental diabetes mellitus that may be predictive of subsequent nephropathy. In the streptozotocin diabetic rat kidney growth rapidly follows the induction of experimental diabetes but the mechanisms responsible for this growth are poorly understood. Epidermal growth factor (EGF) is a potent mitogen for renal tubular cells. Thirty one male Sprague-Dawley rats aged 13 weeks were randomised to receive either streptozotocin (diabetic, n = 20) or buffer (control, n = 11). Animals were studied on days 1, 3, 5 and 7 following streptozotocin. Diabetes was associated with a 3-fold increase in urinary EGF excretion (223 +/- 15 vs 59 +/- 5 ng/day, mean +/- SEM, diabetic vs control, p < 0.0001) and 3-6 fold increase in renal EGF mRNA relative to controls (p < 0.001). A transient rise in kidney EGF protein was noted on day 1. There was no difference between diabetic and control animals with regard to intrarenal sites of EGF expression or in plasma EGF. These data suggest that the increased urinary EGF excretion in diabetic animals is the result of enhanced local production and that EGF is not stored for a prolonged period within renal tubular cells but is released following its synthesis. In the context of the known intrarenal actions of EGF this growth factor may play a role in the pathogenesis of diabetes related kidney growth.
Gov't Doc #: 9243098
DOI: 10.1007/s001250050749
Type: Journal Article
Subjects: Animals
Body Weight
Diabetes Mellitus, Experimental.metabolism.physiopathology
Epidermal Growth Factor.biosynthesis.urine
In Situ Hybridization
Kidney Cortex.metabolism
Kidney Medulla.metabolism
Organ Size
RNA, Messenger.biosynthesis
Rats, Sprague-Dawley
Reference Values
Time Factors
Transcription, Genetic
Appears in Collections:Journal articles

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