Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/12796
Title: A Versatile Approach for the Site-Specific Modification of Recombinant Antibodies Using a Combination of Enzyme-Mediated Bioconjugation and Click Chemistry.
Austin Authors: Alt, Karen;Paterson, Brett M;Westein, Erik;Rudd, Stacey E;Poniger, Stan S;Jagdale, Shweta;Ardipradja, Katie;Connell, Timothy U;Krippner, Guy Y;Nair, Ashish K N;Wang, Xiaowei;Tochon-Danguy, Henri J ;Donnelly, Paul S;Peter, Karlheinz;Hagemeyer, Christoph E
Affiliation: Vascular Biotechnology, Baker IDI, Melbourne (Australia).
Atherothrombosis and Vascular Biology, Baker IDI, Melbourne (Australia).
School of Chemistry/Bio21 Institute, University of Melbourne (Australia).
Vascular Biotechnology, Baker IDI, Melbourne (Australia).
Department of Molecular Imaging and Therapy, Austin Health, Melbourne (Australia).
School of Chemistry/Bio21 Institute, University of Melbourne (Australia).
Vascular Biotechnology, Baker IDI, Melbourne (Australia).
Issue Date: 11-May-2015
Publication information: Angewandte Chemie (international Ed. in English) 2015; 54(26): 7515-9
Abstract: A unique two-step modular system for site-specific antibody modification and conjugation is reported. The first step of this approach uses enzymatic bioconjugation with the transpeptidase Sortase A for incorporation of strained cyclooctyne functional groups. The second step of this modular approach involves the azide-alkyne cycloaddition click reaction. The versatility of the two-step approach has been exemplified by the selective incorporation of fluorescent dyes and a positron-emitting copper-64 radiotracer for fluorescence and positron-emission tomography imaging of activated platelets, platelet aggregates, and thrombi, respectively. This flexible and versatile approach could be readily adapted to incorporate a large array of tailor-made functional groups using reliable click chemistry whilst preserving the activity of the antibody or other sensitive biological macromolecules.
Gov't Doc #: 25962581
URI: https://ahro.austin.org.au/austinjspui/handle/1/12796
DOI: 10.1002/anie.201411507
Journal: Angewandte Chemie (International ed. in English)
URL: https://pubmed.ncbi.nlm.nih.gov/25962581
Type: Journal Article
Subjects: PET imaging
Sortase A
bioconjugation
click chemistry
fluorescence imaging
Appears in Collections:Journal articles

Show full item record

Page view(s)

74
checked on Nov 26, 2024

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.