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Title: Hyperexpression of α-hemolysin explains enhanced virulence of sequence type 93 community-associated methicillin-resistant Staphylococcus aureus.
Austin Authors: Chua, Kyra Y L ;Monk, Ian R;Lin, Ya-Hsun;Seemann, Torsten;Tuck, Kellie L;Porter, Jessica L;Stepnell, Justin;Coombs, Geoffrey W;Davies, John K;Stinear, Timothy P;Howden, Benjamin P 
Affiliation: Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria 3052, Australia
Issue Date: 10-Feb-2014
Publication information: BMC Microbiology 2014; 14(): 31
Abstract: The community-associated methicillin-resistant S. aureus (CA-MRSA) ST93 clone is becoming dominant in Australia and is clinically highly virulent. In addition, sepsis and skin infection models demonstrate that ST93 CA-MRSA is the most virulent global clone of S. aureus tested to date. While the determinants of virulence have been studied in other clones of CA-MRSA, the basis for hypervirulence in ST93 CA-MRSA has not been defined.Here, using a geographically and temporally dispersed collection of ST93 isolates we demonstrate that the ST93 population hyperexpresses key CA-MRSA exotoxins, in particular α-hemolysin, in comparison to other global clones. Gene deletion and complementation studies, and virulence comparisons in a murine skin infection model, showed unequivocally that increased expression of α-hemolysin is the key staphylococcal virulence determinant for this clone. Genome sequencing and comparative genomics of strains with divergent exotoxin profiles demonstrated that, like other S. aureus clones, the quorum sensing agr system is the master regulator of toxin expression and virulence in ST93 CA-MRSA. However, we also identified a previously uncharacterized AraC/XylS family regulator (AryK) that potentiates toxin expression and virulence in S. aureus.These data demonstrate that hyperexpression of α-hemolysin mediates enhanced virulence in ST93 CA-MRSA, and additional control of exotoxin production, in particular α-hemolysin, mediated by regulatory systems other than agr have the potential to fine-tune virulence in CA-MRSA.
DOI: 10.1186/1471-2180-14-31
Journal: BMC Microbiology
Type: Journal Article
Subjects: Animals
Bacterial Toxins.biosynthesis.genetics
Community-Acquired Infections.microbiology.pathology
Disease Models, Animal
Gene Deletion
Gene Expression
Gene Expression Regulation, Bacterial
Genetic Complementation Test
Genome, Bacterial
Hemolysin Proteins.biosynthesis.genetics
Methicillin-Resistant Staphylococcus aureus.isolation & purification.pathogenicity
Mice, Inbred BALB C
Sequence Analysis, DNA
Staphylococcal Skin Infections.microbiology.pathology
Appears in Collections:Journal articles

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