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Title: Fully automated synthesis and coupling of [(18) F]FBEM to glutathione using the iPHASE FlexLab module.
Austin Authors: Ackermann, Uwe ;Plougastel, Lucie;Wichmann, Christian;Goh, Yit Wooi;Yeoh, Shinn Dee;Poniger, Stan S;Tochon-Danguy, Henri J ;Scott, Andrew M 
Affiliation: The University of Melbourne, Parkville, Melbourne, Australia
Ludwig Institute for Cancer Research, Melbourne Branch, Melbourne, Australia
Department of Nuclear Medicine and Centre for PET, Austin Health, Heidelberg, Melbourne, Australia
Issue Date: 7-Jan-2014
Publication information: Journal of Labelled Compounds & Radiopharmaceuticals 2014; 57(2): 115-20
Abstract: Site-specific radiolabelling of peptides or antibodies using [(18) F]FBEM is often preferred over non-site-specific radiolabelling with [(18) F]SFB because it does not affect the affinity of the antibody to its target. Unfortunately, the synthesis of [(18) F]FBEM and its conjugation to thiol containing macromolecules requires some manual intervention, which leads to radiation exposure of the radiochemist. In this publication, we report on the complete automation of [(18) F]FBEM production and its subsequent conjugation to glutathione using a slightly modified iPHASE FlexLab module. [(18) F]FBEM was produced in 1.185 ± 0.168 GBq (15-20%; n = 10; 0.75 ± 0.106 GBq non-decay corrected) with a specific activity of 57 ± 10 GBq/µmol. Radiochemical purity was 97 ± 1% and the synthesis time including HPLC purification and reformulation was 70 min. After evaporation to dryness, [(18) F]FBEM was conjugated to glutathione in PBS buffer pH 7.4 in quantitative yields. This fully automated method does not require any manual intervention and therefore reduces the radiation exposure to the operator.
Gov't Doc #: 24395455
DOI: 10.1002/jlcr.3175
Journal: Journal of labelled compounds & radiopharmaceuticals
Type: Journal Article
Subjects: FBEM
peptide labelling
radiolabelled synthons
Automation, Laboratory
Glutathione.chemical synthesis
Isotope Labeling.methods
Maleimides.chemical synthesis
Radiopharmaceuticals.chemical synthesis
Appears in Collections:Journal articles

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