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Title: Absence of the lysosomal protein Limp-2 attenuates renal injury in crescentic glomerulonephritis.
Austin Authors: Lee, Darren Hiu Kwong;Gan, Poh-Yi;Katerelos, Marina ;Fraser, Scott Andrew;Gleich, Kurt;Holdsworth, Stephen Roger;Power, David Anthony
Affiliation: Department of Nephrology, Austin Health, Heidelberg, Victoria, Australia
Department of Medicine, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia
Institute for Breathing and Sleep, Austin Health, Heidelberg, Victoria, Australia
Department of Medicine, Monash Medical Centre, Centre of Inflammatory Diseases, Monash University, Melbourne, Victoria, Australia
Department of Renal Medicine, Faculty of Medicine, Nursing and Health Sciences, Eastern Health Clinical School, Monash University, Melbourne, Victoria, Australia
Issue Date: 7-Jan-2014
Publication information: Immunology and Cell Biology 2014; 92(5): 400-8
Abstract: In humans, mutations of the intrinsic lysosomal protein SCARB2 are associated with myoclonic epilepsy, collapsing focal and segmental glomerulosclerosis, and tubular proteinuria. Mice with deficiency of Limp-2 (the murine homologue) develop tubular proteinuria but not focal and segmental glomerulosclerosis and they have a defect in macrophage function. To further elucidate the role of Limp-2 in immune function, we induced anti-glomerular basement membrane (GBM) model of crescentic glomerulonephritis in wild-type (WT) and Limp-2(-/-) littermates by intraperitoneal injections of nephrotoxic sheep serum. Renal injury and immune responses were assessed on day 14. Compared with WT, Limp-2(-/-) mice had significantly reduced crescent formation, interstitial inflammation and a trend to reduced tubulointerstitial injury. On day 1 during the heterologous phase of the disease, albuminuria was significantly increased in WT but not in Limp-2(-/-) mice. On day 14, albuminuria and renal function were similar in the two groups. There was, however, a significant reduction in the influx of glomerular macrophages and CD4(+) T cells in Limp-2(-/-) mice. Interleukin (IL)-4 and macrophage chemoattractant protein-1 (MCP-1) mRNA expression levels were significantly reduced. Despite the reduction in numbers of infiltrating cells, flow cytometry showed no difference in macrophage or T-cell numbers in the peripheral blood from untreated mice. The systemic humoral immune response, determined by glomerular mouse immunoglobulin G (IgG) deposition and mouse anti-sheep IgG subclass production, was similar in both groups. These data suggest that absence of Limp-2 reduces inflammation in experimental crescentic glomerulonephritis with decreased macrophage and T-cell infiltration in the kidney. It suggests an important role for Limp-2 in mediating the inflammatory response.
Gov't Doc #: 24394995
DOI: 10.1038/icb.2013.104
Type: Journal Article
Subjects: Albuminuria.etiology
Antigens, CD36.deficiency
Disease Models, Animal
Kidney Function Tests
Lysosome-Associated Membrane Glycoproteins.deficiency
Mice, Knockout
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