Please use this identifier to cite or link to this item:
https://ahro.austin.org.au/austinjspui/handle/1/11866
Title: | Optimal conditions required for influenza A infection-enhanced cross-priming of CD8⁺ T cells specific to cell-associated antigens. | Austin Authors: | Wei, Joe;Waithman, Jason;Xiao, Kun;Oveissi, Sara;Chen, Weisan | Affiliation: | Ludwig Institute for Cancer Research, Melbourne-Austin Branch, Austin Health, Heidelberg, Victoria, Australia | Issue Date: | 10-Sep-2013 | Publication information: | Immunology and Cell Biology 2013; 91(9): 576-82 | Abstract: | Dendritic cells can take up exogenous tumor antigens and present their antigenic epitopes to CD8⁺ T cells (T(CD8⁺)), a process called cross-presentation. Cross-presentation is especially important in antitumor immunity because tumor cells, although carrying tumor antigens, do not activate naive T cells efficiently because of a lack of co-stimulatory molecules. Our group has recently shown that influenza A virus (IAV) infection of allogeneic cells lead to enhanced cross-priming of T(CD8⁺) specific to cellular antigens. To develop this into a potential vaccine strategy, in this study, we have systematically investigated the numbers of allogeneic cells infected by IAV, IAV doses and their infectious activity, the length of in vitro infection and other associated factors. We have defined the optimal immune-enhancing conditions and we have also shown in vivo that such enhanced cross-priming did lead to enhanced tumor protection. The knowledge should be useful for developing more robust cancer vaccine. | Gov't Doc #: | 24018533 | URI: | https://ahro.austin.org.au/austinjspui/handle/1/11866 | DOI: | 10.1038/icb.2013.46 | Journal: | Immunology and cell biology | URL: | https://pubmed.ncbi.nlm.nih.gov/24018533 | Type: | Journal Article | Subjects: | Animals Antigens, Neoplasm.immunology Bystander Effect.immunology CD8-Positive T-Lymphocytes.immunology Cancer Vaccines Cells, Cultured Cross-Priming Dendritic Cells.immunology.virology Influenza A virus.immunology.pathogenicity Melanoma, Experimental.immunology.therapy Membrane Proteins.immunology Mice Mice, Inbred C57BL Neoplasm Transplantation Orthomyxoviridae Infections.immunology |
Appears in Collections: | Journal articles |
Show full item record
Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.