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Title: Comparison of two syngeneic orthotopic murine models of pancreatic adenocarcinoma.
Austin Authors: Nikfarjam, Mehrdad ;Yeo, Dannel;He, Hong ;Baldwin, Graham S;Fifis, Theodora;Costa, Patricia;Tan, Bryan;Yang, Eunice;Wen, Shu wen;Christophi, Christopher 
Affiliation: Department of Surgery, Austin Health, University of Melbourne, Austin Health, LTB8, Heidleberg, Victoria, Australia
Issue Date: 19-Aug-2013
Publication information: Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research 2013; 26(6): 352-9
Abstract: Pancreatic adenocarcinoma has an extremely poor prognosis. The use of appropriate in vivo models is essential in devising methods to improve treatment outcomes.A pancreatic adenocarcinoma model based on tumor injection into the pancreatic head was compared with a pancreatic tail injection model in C57/BL6 mice. The murine pancreatic adenocarcinoma cell line PAN02, dispersed in Matrigelâ„¢, was used for tumor induction.Tumors developed in all animals in both models. Tumor size was more consistent within the pancreatic tail group at 20 days following induction, with no evidence of metastatic disease. Animals in the pancreatic head injection group showed signs of reduced health by 20 days following injection and developed jaundice. Microscopic liver metastases were noted in some of these animals at this time point. The overall survival of animals at 40 days following tumor induction was significantly lower in the pancreatic head injection group (0% vs. 35%; p < .001). Multiple liver metastases were noted in five of 10 (50%) animals in the head injection group, without evidence of peritoneal metastases. In the pancreatic tail injection group, 18 of 20 (90%) animals had multiple peritoneal metastases, and nine of 20 (45%) animals had evidence of isolated liver deposits. Tumors in both regions of the pancreas had similar histologic characteristics, with a dense fibrotic stroma at the interface between the tumor and the normal pancreas.Pancreatic head and tail orthotopic cancer models produce consistent tumors, but the patterns of tumor spread and survival differ according to the site of injection.
Gov't Doc #: 23957638
DOI: 10.3109/08941939.2013.797057
Journal: Journal of investigative surgery : the official journal of the Academy of Surgical Research
Type: Journal Article
Subjects: Adenocarcinoma.etiology.pathology
Cell Line, Tumor
Liver Neoplasms.secondary
Mice, Inbred C57BL
Models, Animal
Neoplasm Transplantation.methods
Pancreatic Neoplasms.etiology.pathology
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