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Title: Foxp3+ T cells in peripheral blood of renal transplant recipients and clinical correlations.
Austin Authors: Lin, Wen X;Christiansen, Dale;Fu, Lu L;Roberts, Matthew A;Sandrin, Mauro S ;Ierino, Francesco L
Affiliation: Department of Surgery, The University of Melbourne, Austin Health/Northern Health, Heidelberg, Victoria, Australia
Issue Date: 1-May-2012
Publication information: Nephrology; 17(4): 415-22
Abstract: Immunophenotype peripheral blood T cells from renal transplant recipients (RTR) using cellular markers of regulatory T cells (Tregs) and flow cytometry, including Foxp3, and correlate these findings with clinical parameters.Expression of phenotypic markers of Tregs was assessed by flow cytometric analysis of peripheral blood lymphocytes (PBL) from (i) RTR (n = 95); (ii) patients with end-stage renal failure (ESRF) awaiting transplantation (n = 17); and (iii) normal healthy controls (n = 18).The percentage of CD4(+) CD25(+) Foxp3(+) cells within the CD4(+) cell population did not significantly alter at different time points post-transplant. However, the percentage of CD4(+) CD25(+) Foxp3(+) cells within the CD4(+) population was significantly lower in RTR compared with patients with ESRF. In contrast, RTR and ESRF had a similar percentage of CD4(+) CD25(+) cells expressing Foxp3. Multivariate analysis of PBL and clinical parameters demonstrated (i) a positive linear relationship between the percentage CD4(+) CD25(+) cells expressing Foxp3 and estimated glomerular filtration rate and (ii) a higher percentage of CD4(+) CD25(+) cells in the CD4(+) cell population in patients with malignancy (the majority were skin cancers). Malignancy also correlated strongly with time post-transplant and age of the RTR.Immune monitoring of the PBL phenotype in RTR using CD4, CD25 and Foxp3 may stratify RTR and predict graft outcome and function, and risk of complications from immunosuppression. Longitudinal and functional studies of Tregs are essential to extend the findings of the present study.
Gov't Doc #: 22308996
DOI: 10.1111/j.1440-1797.2012.01578.x
Journal: Nephrology
Type: Journal Article
Subjects: Adult
Biological Markers.blood
Flow Cytometry
Forkhead Transcription Factors.blood
Glomerular Filtration Rate
Graft Rejection.immunology.prevention & control
Graft Survival
Immunosuppressive Agents.therapeutic use
Interleukin-2 Receptor alpha Subunit.blood
Kidney Failure,
Kidney Transplantation.immunology
Linear Models
Middle Aged
Monitoring, Immunologic
Multivariate Analysis
Risk Assessment
Risk Factors
T-Lymphocytes, Regulatory.drug effects.immunology
Time Factors
Treatment Outcome
Waiting Lists
Appears in Collections:Journal articles

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