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Title: Hyperbaric oxygen therapy reduces the severity of ischaemia, preservation and reperfusion injury in a rat model of liver transplantation.
Austin Authors: Tran, Nhut Quang;Malcontenti-Wilson, Caterina;Hammoud, Soukena;Millar, Ian;Christophi, Christopher ;Muralidharan, Vijayaragavan 
Affiliation: Department of Surgery, The University of Melbourne, Austin Health, Studley Road, Heidelberg, Victoria, Australia
Issue Date: 13-Nov-2011
Publication information: Hpb : the Official Journal of the International Hepato Pancreato Biliary Association 2011; 14(2): 103-14
Abstract:   Approaches to increase organ availability for orthotopic liver transplantation (OLT) often result in the procurement of marginal livers that are more susceptible to ischaemia, preservation and reperfusion injury (IPRI).  The effects of post-OLT hyperbaric oxygen (HBO) therapy on IPRI in a syngeneic rat OLT model were examined at various time-points. The effects of IPRI and HBO on hepatocyte necrosis, apoptosis, proliferation, and sinusoidal morphology and ultrastructure were assessed.  Post-OLT HBO therapy significantly reduced the severity of IPRI; both apoptosis [at 12 h: 6.4 ± 0.4% in controls vs. 1.6 ± 0.7% in the HBO treatment group (p < 0.001); at 48 h: 2.4 ± 0.2% in controls vs. 0.4 ± 0.1% in the HBO treatment group (p < 0.001)] and necrosis [at 12 h: 18.7 ± 1.8% in controls vs. 2.4 ± 0.4% in the HBO treatment group (p < 0.001); at 48 h: 8.5 ± 1.3% in controls vs. 3.4 ± 0.9% in the HBO treatment group (P= 0.019)] were decreased. Serum alanine transaminase was reduced [at 12 h: 1068 ± 920 IU/l in controls vs. 370 ± 63 IU/l in the HBO treatment group (P= 0.030); at 48 h: 573 ± 261 IU/l in controls vs. 160 ± 10 IU/l in the HBO treatment group (P= 0.029)]. Treatment with HBO also promoted liver regeneration [proliferation at 12 h: 4.5 ± 0.1% in controls vs. 1.0 ± 0.3% in the HBO treatment group (p < 0.001); at 48 h: 8.6 ± 0.7% in controls vs. 2.9 ± 0.2% in the HBO treatment group (p < 0.01)] and improved sinusoidal diameter and microvascular density index.  Hyperbaric oxygen therapy has persistent positive effects post-OLT that may potentially transfer into clinical practice.
Gov't Doc #: 22221571
DOI: 10.1111/j.1477-2574.2011.00410.x
Type: Journal Article
Subjects: Animals
Biological Markers.blood
Cell Proliferation
Disease Models, Animal
Hyperbaric Oxygenation
Liver Regeneration
Liver Transplantation.adverse effects
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Rats, Inbred Lew
Reperfusion Injury.blood.etiology.pathology.prevention & control
Severity of Illness Index
Time Factors
Appears in Collections:Journal articles

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