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Title: Antibody activity in ankylosing spondylitis sera to two sites on HLA B27.1 at the MHC groove region (within sequence 65-85), and to a Klebsiella pneumoniae nitrogenase reductase peptide (within sequence 181-199).
Austin Authors: Ewing, C;Ebringer, R;Tribbick, G;Geysen, H M
Affiliation: Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria.
Issue Date: 1-May-1990
Publication information: The Journal of Experimental Medicine; 171(5): 1635-47
Abstract: 74 overlapping peptides of varying lengths from Klebsiella pneumoniae nitrogenase reductase (residues 181-199) and from the HLA B27.1 molecule (residues 65-85) were synthesized and tested by ELISA against sera from HLA B27+ ankylosing spondylitis (AS) patients, and sera from HLA B27+ and HLA B27- healthy first-degree relatives. Antibody activity in AS sera to Klebsiella peptides of four to eight amino acids was maximal with the peptide NSRQTDR. Activity to HLA B27 peptides was maximal with the peptide KAKAQTDR (named epitope I). These peptides overlap with, but are proximal to the NH2 terminus from QTDRED, which is homologous in HLA B27.1 and K. pneumoniae nitrogenase reductase. A second weaker reactive site was noted in the HLA B27.1 peptides, proximal to the COOH terminus from the homologous sequence, namely peptide REDLRTLL (named epitope II). Little activity was seen against peptides that included the entire homologous sequence. Sera from 50 AS patients showed higher total Ig activity against peptides KAKAQTDR (p less than 0.001) and NSRQTDR (p less than 0.02) than did sera from 22 B27+ and 22 B27- healthy controls. These data indicate that AS patient sera contain antibodies that bind to K. pneumoniae nitrogenase peptides and HLA B27.1 peptides, and that there are at least two epitopes on HLA B27.1 in the alpha 1 domain, at the MHC groove region, that are autoantigenic in AS patients. Epitope I may be a site for crossreactivity between HLA B27 and Klebsiella.
Gov't Doc #: 2185331
Type: Journal Article
Subjects: Amino Acid Sequence
Antibodies, Bacterial.immunology
Enzyme-Linked Immunosorbent Assay
HLA-B27 Antigen.immunology
Klebsiella pneumoniae.enzymology.immunology
Molecular Sequence Data
Reference Values
Sequence Homology, Nucleic Acid
Spondylitis, Ankylosing.blood.immunology
Appears in Collections:Journal articles

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