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Title: The regulatory T cell-associated transcription factor FoxP3 is expressed by tumor cells.
Austin Authors: Ebert, Lisa M;Tan, Bee Shin;Browning, Judy;Svobodova, Suzanne;Russell, Sarah E;Kirkpatrick, Naomi;Gedye, Craig;Moss, Denis;Ng, Sweet Ping ;MacGregor, Duncan;Davis, Ian D;Cebon, Jonathan S ;Chen, Weisan
Ludwig Institute for Cancer Research (Melbourne Center for Clinical Sciences), Austin Health, Heidelberg, Victoria, Australia
Issue Date: 15-Apr-2008
Publication information: Cancer Research; 68(8): 3001-9
Abstract: FoxP3 is a member of the forkhead family of transcription factors critically involved in the development and function of CD25(+) regulatory T cells (Treg). Until recently, FoxP3 expression was thought to be restricted to the T-cell lineage. However, using immunohistochemistry and flow cytometric analysis of human melanoma tissue, we detected FoxP3 expression not only in the tumor infiltrating Treg but also in the melanoma cells themselves. FoxP3 is also widely expressed by established human melanoma cell lines (as determined by flow cytometry, PCR, and Western blot), as well as cell lines derived from other solid tumors. Normal B cells do not express FoxP3; however, expression could be induced after transformation with EBV in vitro and in vivo, suggesting that malignant transformation of healthy cells can induce FoxP3. In addition, a FOXP3 mRNA variant lacking exons 3 and 4 was identified in tumor cell lines but was absent from Treg. Interestingly, this alternative splicing event introduces a translation frame-shift that is predicted to encode a novel protein. Together, our results show that FoxP3, a key regulator of immune suppression, is not only expressed by Treg but also by melanoma cells, EBV-transformed B cells, and a wide variety of tumor cell lines.
Gov't Doc #: 18413770
DOI: 10.1158/0008-5472.CAN-07-5664
Type: Journal Article
Subjects: B-Lymphocytes.immunology.virology
Cell Line, Tumor
Flow Cytometry
Forkhead Transcription Factors.genetics.immunology
Herpesvirus 4, Human.immunology
Lymphocyte Activation.immunology
Prostatic Neoplasms.genetics.immunology
T-Lymphocytes, Regulatory.immunology
Appears in Collections:Journal articles

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