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Title: | [125I]Ifenprodil: a convenient radioligand for binding and autoradiographic studies of the polyamine-sensitive site of the NMDA receptor. | Austin Authors: | Beart, P M;Mercer, L D;Jarrott, B | Affiliation: | University of Melbourne, Clinical Pharmacology and Therapeutics Unit, Austin Hospital, Heidelberg, Vic. Australia | Issue Date: | 1-Apr-1991 | Publication information: | Neuroscience Letters; 124(2): 187-9 | Abstract: | Iodination of ifenprodil, a non-competitive NMDA antagonist, with Na125I/Chloramin-T gave a radioligand which bound rapidly and saturably to a single population of sites (dissociation constant 145 nM) in membranes of rat cerebral cortex. In competition studies, specific binding of [125I]-ifenprodil was inhibited by analogues of ifenprodil, as well as by spermine and spermidine. Binding was sensitive to Ca2+, Mg2+ and Zn2+. [125I]-Ifenprodil labelled a population of binding sites, which was topographically distributed in rat forebrain, as shown by autoradiography. [125I]Ifenprodil is a useful radioligand for the investigation of the polyamine site of the N-methyl-D-aspartate (NMDA) receptor-complex. | Gov't Doc #: | 1829796 | URI: | https://ahro.austin.org.au/austinjspui/handle/1/10542 | Journal: | Neuroscience letters | URL: | https://pubmed.ncbi.nlm.nih.gov/1829796 | Type: | Journal Article | Subjects: | Animals Autoradiography Biogenic Polyamines.pharmacology Calcium.pharmacology Cerebral Cortex.drug effects.metabolism Iodine Radioisotopes.diagnostic use Isotope Labeling Magnesium.pharmacology Piperidines.chemical synthesis.diagnostic use Rats Receptors, N-Methyl-D-Aspartate.drug effects Spermidine.pharmacology Zinc.pharmacology |
Appears in Collections: | Journal articles |
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