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Title: | Effect of age on endothelin-1 binding sites in rat cardiac ventricular membranes. | Austin Authors: | Gu, X H;Ou, R C;Casley, David J;Daly, Mark J;Nayler, W G | Affiliation: | Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Australia | Issue Date: | 1-May-1992 | Publication information: | Journal of Cardiovascular Pharmacology; 19(5): 764-9 | Abstract: | To establish whether the density, affinity, or selectivity of endothelin-1 (ET-1) binding sites in cardiac ventricular membranes varies with age, membranes were harvested from 5- to 7-day-, 20-day-, and 8- to 9-week-old Sprague Dawley rats and labeled with [125I]ET-1. Selectivity was established by using cold ET-1, ET-2, ET-3, big ET-1, and (+)PN200-110 to inhibit specific binding of [125I]ET-1. Over the age span studied, selectivity and affinity of the [125I]ET-1 binding sites was unchanged, but density (Bmax) decreased from 209.7 +/- 18.4 at 5-7 days to 154.0 +/- 8.9 (p less than 0.02) at 20 days, and to 89.7 +/- 5.2 (p less than 0.01) fmol/mg protein at 8-9 weeks. These age-dependent differences in Bmax were not accompanied by a change in membrane yield and occurred at a time when the specific binding of (+)[3H]PN200-110 increased. | Gov't Doc #: | 1381775 | URI: | https://ahro.austin.org.au/austinjspui/handle/1/9588 | Journal: | Journal of Cardiovascular Pharmacology | URL: | https://pubmed.ncbi.nlm.nih.gov/1381775 | Type: | Journal Article | Subjects: | Aging.metabolism Animals Binding Sites Binding, Competitive Calcium.antagonists & inhibitors Dihydropyridines.metabolism.pharmacology Endothelin-1 Endothelins.metabolism.pharmacology Female Heart Ventricles.metabolism Isradipine Membranes.metabolism Myocardium.metabolism Ouabain.pharmacology Protein Precursors.metabolism.pharmacology Rats Rats, Inbred Strains Receptors, Cell Surface.metabolism Receptors, Endothelin Sodium-Potassium-Exchanging ATPase.metabolism |
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