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Title: Effect of age on endothelin-1 binding sites in rat cardiac ventricular membranes.
Authors: Gu, X H;Ou, R C;Casley, David J;Daly, Mark J;Nayler, W G
Affiliation: Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Australia.
Issue Date: 1-May-1992
Citation: Journal of Cardiovascular Pharmacology; 19(5): 764-9
Abstract: To establish whether the density, affinity, or selectivity of endothelin-1 (ET-1) binding sites in cardiac ventricular membranes varies with age, membranes were harvested from 5- to 7-day-, 20-day-, and 8- to 9-week-old Sprague Dawley rats and labeled with [125I]ET-1. Selectivity was established by using cold ET-1, ET-2, ET-3, big ET-1, and (+)PN200-110 to inhibit specific binding of [125I]ET-1. Over the age span studied, selectivity and affinity of the [125I]ET-1 binding sites was unchanged, but density (Bmax) decreased from 209.7 +/- 18.4 at 5-7 days to 154.0 +/- 8.9 (p less than 0.02) at 20 days, and to 89.7 +/- 5.2 (p less than 0.01) fmol/mg protein at 8-9 weeks. These age-dependent differences in Bmax were not accompanied by a change in membrane yield and occurred at a time when the specific binding of (+)[3H]PN200-110 increased.
Internal ID Number: 1381775
Type: Journal Article
Subjects: Aging.metabolism
Binding Sites
Binding, Competitive
Calcium.antagonists & inhibitors
Heart Ventricles.metabolism
Protein Precursors.metabolism.pharmacology
Rats, Inbred Strains
Receptors, Cell Surface.metabolism
Receptors, Endothelin
Sodium-Potassium-Exchanging ATPase.metabolism
Appears in Collections:Journal articles

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