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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Dillon, J S | en |
dc.contributor.author | Gu, X H | en |
dc.contributor.author | Nayler, W G | en |
dc.date.accessioned | 2015-05-16T02:37:25Z | |
dc.date.available | 2015-05-16T02:37:25Z | |
dc.date.issued | 1988-08-01 | en |
dc.identifier.citation | Journal of Molecular and Cellular Cardiology; 20(8): 725-35 | en |
dc.identifier.govdoc | 2851664 | en |
dc.identifier.other | PUBMED | en |
dc.identifier.uri | https://ahro.austin.org.au/austinjspui/handle/1/12874 | en |
dc.description.abstract | The [3H]-prazosin binding activity of membranes isolated from aerobically perfused, ischaemic and reperfused cat and rat hearts was investigated. Alpha 1 adrenoceptors in membranes from aerobically perfused cat hearts had a KD of 0.363 +/- 0.067 nM and a Bmax of 68.8 +/- 7.0 fmol/mg protein; 30 min normothermic global ischaemia caused an increase (P less than 0.01) in density (Bmax, 111.4 +/- 9.3 fmol/mg protein), without any change in affinity (KD 0.430 +/- 0.069 nM). Post-ischaemic reperfusion (15 min) caused the Bmax to return to control values, with no change in KD. Membranes isolated from aerobically perfused rat hearts contained a single population of high affinity alpha 1 adrenoceptor binding sites, with a KD of 0.092 +/- 0.02 nM and a Bmax of 43.02 +/- 2.49 fmol/mg protein. Neither global nor low-flow (0.1 ml/min) ischaemia for either 30 or 60 min, nor post-ischaemic reperfusion, caused any change in either the affinity or density of these binding sites. In both species, and under all the above experimental conditions, the selectivity of the alpha 1 adrenoceptor binding sites was maintained, with phentolamine much greater than rauwolscine in displacing bound [3H]-prazosin. These results show that the ability of ischaemia to increase alpha 1 adrenoceptor density in cardiac membranes is species specific, and that it can occur as a direct (as opposed to a reflex) response to ischaemia. | en |
dc.language.iso | en | en |
dc.subject.other | Aerobiosis | en |
dc.subject.other | Animals | en |
dc.subject.other | Binding, Competitive | en |
dc.subject.other | Cell Membrane.metabolism | en |
dc.subject.other | Coronary Disease.metabolism | en |
dc.subject.other | Kinetics | en |
dc.subject.other | Male | en |
dc.subject.other | Myocardium.metabolism | en |
dc.subject.other | Prazosin.metabolism | en |
dc.subject.other | Rats | en |
dc.subject.other | Rats, Inbred Strains | en |
dc.subject.other | Receptors, Adrenergic, alpha.metabolism | en |
dc.subject.other | Reference Values | en |
dc.title | Alpha 1 adrenoceptors in the ischaemic and reperfused myocardium. | en |
dc.type | Journal Article | en |
dc.identifier.journaltitle | Journal of molecular and cellular cardiology | en |
dc.identifier.affiliation | Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia | en |
dc.description.pages | 725-35 | en |
dc.relation.url | https://pubmed.ncbi.nlm.nih.gov/2851664 | en |
dc.type.austin | Journal Article | en |
item.languageiso639-1 | en | - |
item.openairetype | Journal Article | - |
item.cerifentitytype | Publications | - |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | none | - |
Appears in Collections: | Journal articles |
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