Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/10875
Title: Reduction in renal ACE2 expression in subtotal nephrectomy in rats is ameliorated with ACE inhibition.
Authors: Velkoska, Elena;Dean, Rachael G;Burchill, Luke J;Levidiotis, Vicki;Burrell, Louise M
Affiliation: Department of Medicine, The University of Melbourne, Austin Health, Heidelberg 3081, Victoria, Australia.
Issue Date: 1-Feb-2010
Citation: Clinical Science (london, England : 1979); 118(4): 269-79
Abstract: Alterations within the RAS (renin-angiotensin system) are pivotal for the development of renal disease. ACE2 (angiotensin-converting enzyme 2) is expressed in the kidney and converts the vasoconstrictor AngII (angiotensin II) into Ang-(1-7), a peptide with vasodilatory and anti-fibrotic actions. Although the expression of ACE2 in the diabetic kidney has been well studied, little is known about its expression in non-diabetic renal disease. In the present study, we assessed ACE2 in rats with acute kidney injury induced by STNx (subtotal nephrectomy). STNx and Control rats received vehicle or ramipril (1 mg. kg (-1) of body weight . day (-1), and renal ACE, ACE2 and mas receptor gene and protein expression were measured 10 days later. STNx rats were characterized by polyuria, proteinuria, hypertension and elevated plasma ACE2 activity (all P<0.01) and plasma Ang-(1-7) (P<0.05) compared with Control rats. There was increased cortical ACE binding and medullary mas receptor expression (P<0.05), but reduced cortical and medullary ACE2 activity in the remnant kidney (P<0.05 and P<0.001 respectively) compared with Control rats. In STNx rats, ramipril reduced blood pressure (P<0.01), polyuria (P<0.05)and plasma ACE2 (P<0.01), increased plasma Ang-(1-7) (P<0.001), and inhibited renal ACE(P<0.001). Ramipril increased both cortical and medullary ACE2 activity (P<0.01), but reduced medullary mas receptor expression (P<0.05). In conclusion, our results show that ACE2 activity is reduced in kidney injury and that ACE inhibition produced beneficial effects in association with increased renal ACE2 activity. As ACE2 both degrades AngII and generates the vasodilator Ang-(1-7), a decrease in renal ACE2 activity, as observed in the present study, has the potential to contribute to the progression of kidney disease.
Internal ID Number: 19698082
URI: http://ahro.austin.org.au/austinjspui/handle/1/10875
DOI: 10.1042/CS20090318
URL: http://www.ncbi.nlm.nih.gov/pubmed/19698082
Type: Journal Article
Subjects: Angiotensin-Converting Enzyme Inhibitors.pharmacology
Animals
Female
Gene Expression
Hypertension.drug therapy.metabolism
Kidney.drug effects.injuries.metabolism
Nephrectomy
Peptidyl-Dipeptidase A.metabolism
Polyuria.drug therapy.metabolism
Proteinuria.drug therapy.metabolism
Proto-Oncogene Proteins.genetics.metabolism
Ramipril.pharmacology
Random Allocation
Rats
Rats, Sprague-Dawley
Receptors, G-Protein-Coupled.genetics.metabolism
Appears in Collections:Journal articles

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