Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9847
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dc.contributor.authorBallard, Susan A-
dc.contributor.authorGrabsch, Elizabeth A-
dc.contributor.authorJohnson, Paul D R-
dc.contributor.authorGrayson, M Lindsay-
dc.date.accessioned2015-05-15T23:06:33Z
dc.date.available2015-05-15T23:06:33Z
dc.date.issued2005-01-01-
dc.identifier.citationAntimicrobial Agents and Chemotherapy; 49(1): 77-81en_US
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/9847en
dc.description.abstractWe assessed the sensitivities and specificities of three previously described PCR primers on enrichment broth cultures of feces for the accurate detection of fecal carriage of vancomycin-resistant enterococci (VRE). In addition, we investigated specimens that were vanB PCR positive but VRE culture negative for the presence of other vanB-containing pathogens. Feces from 59 patients (12 patients carrying vanB Enterococcus faecium strains and 47 patients negative for VRE carriage) were cultured for 36 h in aerobic brain heart infusion (BHI) broth, anaerobic BHI (AnO(2)BHI) broth, or aerobic Enterococcosel (EC) broth. DNA was extracted from the cultures and tested for the presence of vanB by using the PCR primers of Dutka-Malen et al. (S. Dutka-Malen, S. Evers, and P. Courvalin, J. Clin. Microbiol. 33:24-27, 1995), Bell et al. (J. M. Bell, J. C. Paton, and J. Turnidge, J. Clin. Microbiol. 36:2187-2190, 1998), and Stinear et al. (T. P. Stinear, D. C. Olden, P. D. R. Johnson, J. K. Davies, and M. L. Grayson, Lancet 357:855-856, 2001). The sensitivity (specificity) of PCR compared with the results of culture on BHI, AnO(2)BHI, and EC broths were 67% (96%), 50% (94%), and 17% (100%), respectively, with the primers of Dutka-Malen et al.; 92% (60%), 92% (45%), and 92% (83%), respectively, with the primers of Bell et al.; and 92% (49%), 92% (43%), and 100% (51%) respectively, with the primers of Stinear et al. The primers of both Bell et al. and Stinear et al. were significantly more sensitive than those of Dutka-Malen et al. in EC broth (P = 0.001 and P < 0.001, respectively). The poor specificities for all primer pairs were due in part to the isolation and identification of six anaerobic gram-positive bacilli, Clostridium hathewayi (n = 3), a Clostridium innocuum-like organism (n = 1), Clostridium bolteae (n = 1), and Ruminococcus lactaris-like (n = 1), from five fecal specimens that were vanB positive but VRE culture negative. All six organisms were demonstrated to contain a vanB gene identical to that of VRE. VanB-containing bowel anaerobes may result in false-positive interpretation of PCR-positive fecal enrichment cultures as VRE, regardless of the primers and protocols used.en_US
dc.language.isoenen
dc.subject.otherBacteria, Anaerobic.geneticsen
dc.subject.otherBacterial Proteins.geneticsen
dc.subject.otherCarrier State.microbiologyen
dc.subject.otherCulture Mediaen
dc.subject.otherDNA Primersen
dc.subject.otherEnterococcus.drug effects.genetics.isolation & purificationen
dc.subject.otherEnterococcus faecium.drug effects.geneticsen
dc.subject.otherFeces.microbiologyen
dc.subject.otherGram-Positive Bacteria.drug effects.geneticsen
dc.subject.otherGram-Positive Bacterial Infections.microbiologyen
dc.subject.otherHumansen
dc.subject.otherMolecular Sequence Dataen
dc.subject.otherPolymerase Chain Reaction.methodsen
dc.subject.otherSensitivity and Specificityen
dc.subject.otherSequence Analysis, DNAen
dc.subject.otherVancomycin Resistance.geneticsen
dc.titleComparison of three PCR primer sets for identification of vanB gene carriage in feces and correlation with carriage of vancomycin-resistant enterococci: interference by vanB-containing anaerobic bacilli.en_US
dc.typeJournal Articleen_US
dc.identifier.journaltitleAntimicrobial Agents and Chemotherapyen_US
dc.identifier.affiliationInfectious Diseasesen_US
dc.identifier.doi10.1128/AAC.49.1.77-81.2005en_US
dc.description.pages77-81en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/15616278en
dc.type.contentTexten_US
dc.type.austinJournal Articleen
local.name.researcherGrabsch, Elizabeth A
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.openairetypeJournal Article-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptInfectious Diseases-
crisitem.author.deptInfectious Diseases-
crisitem.author.deptInfectious Diseases-
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