Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9706
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dc.contributor.authorTrevisin, Men
dc.contributor.authorNeeson, Pen
dc.contributor.authorSavige, Judy Aen
dc.date.accessioned2015-05-15T22:53:56Z
dc.date.available2015-05-15T22:53:56Z
dc.date.issued2004-03-01en
dc.identifier.citationJournal of Clinical Pathology; 57(3): 303-8en
dc.identifier.govdoc14990605en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/9706en
dc.description.abstractThe demonstration of proteinase 3 specific antineutrophil cytoplasmic antibodies (PR3-ANCA), and the estimation of antibody values are useful in the diagnosis and management of patients with Wegener's granulomatosis (WG). However, external quality assessment programmes suggest that PR3-ANCA binding varies in different assays.To demonstrate variations in PR3-ANCA binding in different commercial and in house enzyme linked immunosorbent assays (ELISAs).Binding of a PR3-ANCA standard and 19 sera from patients with WG was compared in eight commercial and in house assays. Binding was expressed in different units depending on the kit.One commercial assay performed unsatisfactorily. Three commercial kits produced PR3-ANCA binding (70, 102, and 84 U/ml) close to the expected value for the standard (100 U/ml). Serial dilutions of this standard were linear in only one commercial assay and the in house assay. Sera from patients with WG with borderline binding in the in house assay bound in the eight commercial kits at 0-148 kit units; low binding sera ranged from 0 to 273 units; moderately strong sera bound at 7-260 units; and strongly binding sera bound at 13-336 units. In four assays, at least one strongly positive serum bound at levels greater than the provided range.Levels of antibody binding and units of binding have not been standardised in commercially available PR3-ANCA ELISAs. This may affect the diagnosis and management of patients with WG, in addition to the implementation of international guidelines for treatment.en
dc.language.isoenen
dc.subject.otherAntibodies, Antineutrophil Cytoplasmic.metabolismen
dc.subject.otherEnzyme-Linked Immunosorbent Assay.methodsen
dc.subject.otherFluorescent Antibody Technique, Indirect.methodsen
dc.subject.otherGranulomatosis with Polyangiitis.diagnosis.metabolismen
dc.subject.otherHumansen
dc.subject.otherMyeloblastinen
dc.subject.otherReagent Kits, Diagnostic.standardsen
dc.subject.otherReproducibility of Resultsen
dc.subject.otherSerine Endopeptidases.metabolismen
dc.titleThe binding of proteinase 3 antineutrophil cytoplasmic antibodies (PR3-ANCA) varies in different ELISAs.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of clinical pathologyen
dc.identifier.affiliationImmunology Unit, Division of Laboratory Medicine, Austin Hospital, Heidelberg, Victoria 3084, Australiaen
dc.description.pages303-8en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/14990605en
dc.type.austinJournal Articleen
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
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