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https://ahro.austin.org.au/austinjspui/handle/1/9368
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gallicchio, M A | en |
dc.contributor.author | Kneen, M | en |
dc.contributor.author | Hall, C | en |
dc.contributor.author | Scott, Andrew M | en |
dc.contributor.author | Bach, Leon A | en |
dc.date.accessioned | 2015-05-15T22:26:20Z | |
dc.date.available | 2015-05-15T22:26:20Z | |
dc.date.issued | 2001-12-01 | en |
dc.identifier.citation | International Journal of Cancer. Journal International Du Cancer; 94(5): 645-51 | en |
dc.identifier.govdoc | 11745458 | en |
dc.identifier.other | PUBMED | en |
dc.identifier.uri | https://ahro.austin.org.au/austinjspui/handle/1/9368 | en |
dc.description.abstract | Rhabdomyosarcoma is the most common soft-tissue sarcoma of childhood. Rhabdomyosarcoma cell lines overexpress insulin-like growth factor-II (IGF-II), an autocrine growth factor that is inhibited by insulin-like growth factor binding protein-6 (IGFBP-6). IGFBP-6 is associated with myoblast quiescence, and expression in rhabdomyosarcoma cells is low. The effect of IGFBP-6 on 2 rhabdomyosarcoma cell lines, RD and Rh30, was studied. IGFBP-6 inhibited anchorage-dependent growth of RD and Rh30 cells in a dose-dependent manner (p < 0.0001). IGFBP-6 also inhibited anchorage-independent growth of RD cells in soft agar in a dose-dependent manner (p < 0.01). Anchorage-independent growth of RD cells on polyhydroxyethylmethacrylate-coated plates was decreased to a minimum of 48% of control after treatment with IGFBP-6 (p < 0.001). In this system, IGFBP-6 increased apoptosis 4-fold (p < 0.001). IGF-II partially reversed the IGFBP-6-induced decrease in growth and increase in apoptosis. Rh30 cells were stably transfected with an IGFBP-6 cDNA and subcutaneous xenografts established in BALB/c nude mice. After 18 days, sizes of 2 independent clones of IGFBP-6-overexpressing Rh30 cells were reduced to 12% and 26% of vector control-transfected tumors (p = 0.0006 and 0.002, respectively). IGFBP-6 therefore inhibits proliferation and promotes apoptosis of rhabdomyosarcoma in vitro and dramatically inhibits xenograft growth in vivo, at least in part by inhibiting IGF-II. Low expression of IGFBP-6 may therefore contribute to rhabdomyosarcoma growth and metastasis. | en |
dc.language.iso | en | en |
dc.subject.other | Animals | en |
dc.subject.other | Apoptosis | en |
dc.subject.other | Cell Division | en |
dc.subject.other | Female | en |
dc.subject.other | Humans | en |
dc.subject.other | Insulin-Like Growth Factor Binding Protein 6.physiology | en |
dc.subject.other | Mice | en |
dc.subject.other | Mice, Inbred BALB C | en |
dc.subject.other | Mice, Nude | en |
dc.subject.other | Rhabdomyosarcoma.pathology.prevention & control | en |
dc.subject.other | Tumor Cells, Cultured | en |
dc.title | Overexpression of insulin-like growth factor binding protein-6 inhibits rhabdomyosarcoma growth in vivo. | en |
dc.type | Journal Article | en |
dc.identifier.journaltitle | International journal of cancer. Journal international du cancer | en |
dc.identifier.affiliation | Department of Medicine, University of Melbourne, Austin and Repatriation Medical Centre (Austin Campus) Heidelberg, Victoria, Australia | en |
dc.description.pages | 645-51 | en |
dc.relation.url | https://pubmed.ncbi.nlm.nih.gov/11745458 | en |
dc.type.austin | Journal Article | en |
local.name.researcher | Scott, Andrew M | |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | none | - |
item.openairetype | Journal Article | - |
item.fulltext | No Fulltext | - |
crisitem.author.dept | Molecular Imaging and Therapy | - |
crisitem.author.dept | Olivia Newton-John Cancer Research Institute | - |
Appears in Collections: | Journal articles |
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