Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9314
Full metadata record
DC FieldValueLanguage
dc.contributor.authorBusuttil, Bridget Een
dc.contributor.authorFrauman, Albert Gen
dc.date.accessioned2015-05-15T22:22:00Z
dc.date.available2015-05-15T22:22:00Z
dc.date.issued2001-05-01en
dc.identifier.citationThe Journal of Clinical Endocrinology and Metabolism; 86(5): 2315-9en
dc.identifier.govdoc11344244en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/9314en
dc.description.abstractThe existence of an autoantigen common to Graves' disease and its associated extrathyroidal manifestations has been proposed. As the TSH receptor (TSHR) is the primary target for autoimmunity against the thyroid in Graves' disease, much effort has gone into investigating the role of TSHR messenger ribonucleic acid (mRNA) transcripts in extrathyroidal tissue, particularly in the orbit. A high stringency RT-PCR technique had previously been employed by our laboratory to demonstrate the presence of full-length and splice variant TSHR mRNA in extraocular muscle (EOM). This technique demonstrated selective amplification of TSHR mRNA transcripts in thyroid and EOM, but not in abdominal muscle, kidney, or brain tissue. We used this technique in the present study to investigate the reported presence of TSHR mRNA transcripts in cardiac tissue. After removal of all visible fat from muscle tissues, high stringency RT-PCR was performed, and we found no TSHR transcripts in the muscle of any chamber of the normal human heart. In addition, in situ hybridization on fresh-frozen and paraffin-embedded sections confirmed the presence of TSHR transcripts in thyroid and EOM, but not in abdominal or cardiac muscle. These findings support the hypothesis that the TSHR is a shared antigen in the thyroid and EOM, but not in cardiac muscle.en
dc.language.isoenen
dc.subject.otherAgeden
dc.subject.otherAutoradiographyen
dc.subject.otherBlotting, Southernen
dc.subject.otherGraves Disease.complications.metabolismen
dc.subject.otherHumansen
dc.subject.otherIn Situ Hybridizationen
dc.subject.otherMaleen
dc.subject.otherMuscles.metabolismen
dc.subject.otherMyocardium.metabolismen
dc.subject.otherRNA, Messenger.analysisen
dc.subject.otherReceptors, Thyrotropin.geneticsen
dc.subject.otherReverse Transcriptase Polymerase Chain Reactionen
dc.titleExtrathyroidal manifestations of Graves' disease: the thyrotropin receptor is expressed in extraocular, but not cardiac, muscle tissues.en
dc.typeJournal Articleen
dc.identifier.journaltitleThe Journal of Clinical Endocrinology and Metabolismen
dc.identifier.affiliationClinical Pharmacology and Therapeutics Unit, Department of Medicine, Austin and Repatriation Medical Center, University of Melbourne, Heidelberg, Victoria 3084, Australiaen
dc.identifier.doi10.1210/jcem.86.5.7474en
dc.description.pages2315-9en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/11344244en
dc.type.austinJournal Articleen
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
crisitem.author.deptClinical Pharmacology and Therapeutics-
Appears in Collections:Journal articles
Show simple item record

Page view(s)

2
checked on Feb 6, 2023

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.