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DC Field | Value | Language |
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dc.contributor.author | Osicka, Tanya M | en |
dc.contributor.author | Houlihan, Christine A | en |
dc.contributor.author | Chan, J Gordon | en |
dc.contributor.author | Jerums, George | en |
dc.contributor.author | Comper, Wayne D | en |
dc.date.accessioned | 2015-05-15T22:15:50Z | |
dc.date.available | 2015-05-15T22:15:50Z | |
dc.date.issued | 2000-09-01 | en |
dc.identifier.citation | Diabetes; 49(9): 1579-84 | en |
dc.identifier.govdoc | 10969843 | en |
dc.identifier.other | PUBMED | en |
dc.identifier.uri | https://ahro.austin.org.au/austinjspui/handle/1/9245 | en |
dc.description.abstract | Previous studies by our group have shown that albumin is metabolized in rodents during renal passage and excreted in the urine as a mixture of intact protein and albumin-derived fragments. The aim of this study was to examine whether albumin is metabolized during renal passage in nondiabetic volunteers and in type 1 diabetic patients with varying levels of albuminuria. Nine nondiabetic normoalbuminuric volunteers and 11 type 1 diabetic patients with albumin excretion rates varying from normoalbuminuria to macroalbuminuria were studied. Each subject received an intravenous injection of tritium-labeled albumin ([3H]-albumin). Urine was collected at 4 h and 24 h after injection and analyzed by size exclusion chromatography. The amount of intact and fragmented albumin was quantified, and each fraction was analyzed by radioimmunoassay (RIA) for albumin. [3H]-albumin in nondiabetic volunteers was metabolized during renal passage to small peptide fragments not detectable by conventional RIA (only 0.05-3.8% of the total urinary radioactivity was associated with intact albumin). The process responsible for albumin fragmentation was similar in diabetic patients with normoalbuminuria (intact albumin represented 0.01-4.0% of total urinary radioactivity). However, there was a reduction in the fragmentation ratio (fragmented:intact) in diabetic patients with micro- or macroalbuminuria (intact albumin represented 2.7-55.5%, P = 0.048). This change in the fragmentation ratio was directly related to the degree of albuminuria. These results have important implications for understanding the mechanisms underlying albuminuria in nondiabetic volunteers and type 1 diabetic patients. In nondiabetic volunteers, the renal processing of albumin involves a relatively rapid and comprehensive degradation of albumin to small fragments (range 1-15 kDa). The degradation process is inhibited in diabetic nephropathy in proportion to the level of albuminuria detected by RIA. | en |
dc.language.iso | en | en |
dc.subject.other | Adult | en |
dc.subject.other | Aged | en |
dc.subject.other | Albuminuria | en |
dc.subject.other | Diabetes Mellitus, Type 1.physiopathology.urine | en |
dc.subject.other | Diabetic Nephropathies.physiopathology.urine | en |
dc.subject.other | Female | en |
dc.subject.other | Humans | en |
dc.subject.other | Kidney.physiopathology | en |
dc.subject.other | Lysosomes.metabolism | en |
dc.subject.other | Male | en |
dc.subject.other | Middle Aged | en |
dc.subject.other | Reference Values | en |
dc.subject.other | Serum Albumin.metabolism | en |
dc.subject.other | Tritium | en |
dc.title | Albuminuria in patients with type 1 diabetes is directly linked to changes in the lysosome-mediated degradation of albumin during renal passage. | en |
dc.type | Journal Article | en |
dc.identifier.journaltitle | Diabetes | en |
dc.identifier.affiliation | Department of Medicine, Austin & Repatriation Medical Center, University of Melbourne, Heidelberg, Victoria, Australia | en |
dc.description.pages | 1579-84 | en |
dc.relation.url | https://pubmed.ncbi.nlm.nih.gov/10969843 | en |
dc.type.austin | Journal Article | en |
local.name.researcher | Chan, J Gordon | |
item.languageiso639-1 | en | - |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.openairetype | Journal Article | - |
crisitem.author.dept | Endocrinology | - |
crisitem.author.dept | Molecular Imaging and Therapy | - |
crisitem.author.dept | Endocrinology | - |
Appears in Collections: | Journal articles |
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