Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9215
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dc.contributor.authorLiu, J Jen
dc.contributor.authorPeng, Len
dc.contributor.authorBradley, C Jen
dc.contributor.authorZulli, Anthonyen
dc.contributor.authorShen, Jen
dc.contributor.authorBuxton, Brian Fen
dc.date.accessioned2015-05-15T22:13:24Z
dc.date.available2015-05-15T22:13:24Z
dc.date.issued2000-02-01en
dc.identifier.citationCardiovascular Research; 45(3): 729-35en
dc.identifier.govdoc10728395en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/9215en
dc.description.abstractThe present studies were undertaken to identify apoptosis in cardiomyocytes of genetic hypertension and to study the relationship among apoptosis, aging and blood pressure, and the effect of angiotensin-converting enzyme (ACE) inhibitors on apoptosis.Apoptosis in the hearts of spontaneously hypertensive rats (SHR) was identified by electron microscopy (EM) and DNA laddering, and quantified from age 3 weeks to 64 weeks in comparison with normotensive rats (WKY). Fibroblasts and protein products of Bcl-2 and Bax were measured by quantitative immunohistochemistry. SHR were treated with ramipril, an ACE inhibitor.The results showed that: (1) ultrastructural characteristics of apoptosis were observed in cardiomyocytes of SHR, with shrinkage of the cell and condensation of the cytoplasm and chromatin. A DNA ladder was shown; (2) a significant increase in apoptosis in SHR began as early as age 4 weeks and reached a plateau at 16 weeks and maintained at high levels up to 64 weeks. Blood pressure (BP) in SHR started to increase significantly at age 5 weeks; (3) fibroblasts were significantly increased in the heart of SHR; (4) the ratio of Bcl-2/Bax was significantly reduced in SHR; and (6) ramipril effectively reduced apoptosis and fibroblasts, and increased the ratio of Bcl-2/Bax.Apoptosis occurs in the cardiomyocytes of genetic hypertension although fibroblasts are increased, and a significant, age-dependent increase in apoptosis is observed. The increase in apoptosis occurs before the difference in blood pressure is detectable. The ACE inhibitor ramipril may be useful for prevention of apoptosis in the heart.en
dc.language.isoenen
dc.subject.otherAnalysis of Varianceen
dc.subject.otherAngiotensin-Converting Enzyme Inhibitors.therapeutic useen
dc.subject.otherAnimalsen
dc.subject.otherApoptosis.drug effectsen
dc.subject.otherDNA Fragmentationen
dc.subject.otherFibroblasts.pathologyen
dc.subject.otherHeart.physiopathologyen
dc.subject.otherHypertension.genetics.pathology.physiopathologyen
dc.subject.otherMaleen
dc.subject.otherMicroscopy, Electronen
dc.subject.otherProto-Oncogene Proteins.metabolismen
dc.subject.otherProto-Oncogene Proteins c-bcl-2.metabolismen
dc.subject.otherRamipril.therapeutic useen
dc.subject.otherRatsen
dc.subject.otherRats, Inbred WKYen
dc.subject.otherbcl-2-Associated X Proteinen
dc.titleIncreased apoptosis in the heart of genetic hypertension, associated with increased fibroblasts.en
dc.typeJournal Articleen
dc.identifier.journaltitleCardiovascular researchen
dc.identifier.affiliationCardiovascular Biology Unit, Austin Hospital, University of Melbourne, Heidelberg, Victoria, Australiaen
dc.description.pages729-35en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/10728395en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.languageiso639-1en-
item.openairetypeJournal Article-
crisitem.author.deptCardiac Surgery-
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