Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9147
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dc.contributor.authorMcKenzie, Ian F Cen
dc.contributor.authorPatton, Ken
dc.contributor.authorSmit, J Aen
dc.contributor.authorMouhtouris, Een
dc.contributor.authorXing, Pei Xiangen
dc.contributor.authorMyburgh, Johnen
dc.contributor.authorSandrin, Mauro Sen
dc.date.accessioned2015-05-15T22:07:26Z
dc.date.available2015-05-15T22:07:26Z
dc.date.issued1999-03-27en
dc.identifier.citationTransplantation; 67(6): 864-70en
dc.identifier.govdoc10199735en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/9147en
dc.description.abstractThe Gal alpha(1,3)Gal epitope is of interest as, in pig-to-primate xenotransplantation, it is the major target of naturally occurring human IgM and IgG antibodies, leading to hyperacute rejection. Human and Old World monkeys make anti-Gal alpha(1,3)Gal antibodies as they lack a functional gene and do not express Gal alpha(1,3)Gal. Interestingly, the cultured fibroblasts of some other species, such as chickens, have been reported also not to express Gal alpha(1,3)Gal--if this is true for other tissues, and chickens do not express Gal alpha(1,3)Gal antigen, then they would have anti-Gal antibodies--which could have diagnostic and therapeutic value, particularly as chicken antibodies do not fix mammalian complement.Standard serological methods were used to characterize the antibodies. Several baboons received pig kidney xenografts that had been perfused with hyperimmune chicken anti-Gal antibodies.We now demonstrate that chickens do not express Gal alpha(1,3)Gal on their red cells, leukocytes, or tissues, and that their serum contains large amounts of anti-Gal alpha(1,3)Gal antibodies. In addition, chickens could be immunized to produce high-titer, high-avidity antibodies (9.5x10(9) M(-1))--an avidity considerably greater than that of the Gal alpha(1,3)Gal binding lectin IB4 (2.9x10(8) M(-1)) or Gal antibodies in human serum (2.2x10(5) M(-1)). Chicken antibodies, obtained from both normal and immunized chickens, could block the in vitro cytolysis of pig endothelial cells or lymphocytes by human or baboon antibodies. However, such antibodies tested in vivo in pig-to-baboon xenotransplantation failed to block hyperacute rejection and, indeed, may have accelerated this.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherAntibodies.immunologyen
dc.subject.otherAntibody Affinityen
dc.subject.otherBinding, Competitiveen
dc.subject.otherCOS Cellsen
dc.subject.otherChickens.immunologyen
dc.subject.otherDisaccharides.immunologyen
dc.subject.otherEpitopesen
dc.subject.otherHumansen
dc.subject.otherImmunizationen
dc.subject.otherKidney Transplantationen
dc.subject.otherPapioen
dc.subject.otherSwineen
dc.titleDefinition and characterization of chicken Gal alpha(1,3)Gal antibodies.en
dc.typeJournal Articleen
dc.identifier.journaltitleTransplantationen
dc.identifier.affiliationMolecular Immunogenetics Laboratory, Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australiaen
dc.description.pages864-70en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/10199735en
dc.type.austinJournal Articleen
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
crisitem.author.deptInfectious Diseases-
crisitem.author.deptSurgery (University of Melbourne)-
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