Please use this identifier to cite or link to this item:
https://ahro.austin.org.au/austinjspui/handle/1/34004
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DC Field | Value | Language |
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dc.contributor.author | Higgs, Charlie | - |
dc.contributor.author | Kumar, Lamali Sadeesh | - |
dc.contributor.author | Stevens, Kerrie | - |
dc.contributor.author | Strachan, Janet | - |
dc.contributor.author | Korman, Tony | - |
dc.contributor.author | Horan, Kristy | - |
dc.contributor.author | Daniel, Diane | - |
dc.contributor.author | Russell, Madeline | - |
dc.contributor.author | McDevitt, Christopher A | - |
dc.contributor.author | Sherry, Norelle L | - |
dc.contributor.author | Stinear, Timothy P | - |
dc.contributor.author | Howden, Benjamin P | - |
dc.contributor.author | Gorrie, Claire L | - |
dc.date | 2023 | - |
dc.date.accessioned | 2023-10-18T03:29:34Z | - |
dc.date.available | 2023-10-18T03:29:34Z | - |
dc.date.issued | 2023-11-15 | - |
dc.identifier.citation | Antimicrobial Agents and Chemotherapy 2023-11-15; 67(11) | en_US |
dc.identifier.issn | 1098-6596 | - |
dc.identifier.uri | https://ahro.austin.org.au/austinjspui/handle/1/34004 | - |
dc.description.abstract | Streptococcus pneumoniae is a major human pathogen with a high burden of disease. Non-invasive isolates (those found in non-sterile sites) are thought to be a key source of invasive isolates (those found in sterile sites) and a reservoir of anti-microbial resistance (AMR) determinants. Despite this, pneumococcal surveillance has almost exclusively focused on invasive isolates. We aimed to compare contemporaneous invasive and non-invasive isolate populations to understand how they interact and identify differences in AMR gene distribution. We used a combination of whole-genome sequencing and phenotypic anti-microbial susceptibility testing and a data set of invasive (n = 1,288) and non-invasive (n = 186) pneumococcal isolates, collected in Victoria, Australia, between 2018 and 2022. The non-invasive population had increased levels of antibiotic resistance to multiple classes of antibiotics including beta-lactam antibiotics penicillin and ceftriaxone. We identified genomic intersections between the invasive and non-invasive populations and no distinct phylogenetic clustering of the two populations. However, this analysis revealed sub-populations overrepresented in each population. The sub-populations that had high levels of AMR were overrepresented in the non-invasive population. We determined that WamR-Pneumo was the most accurate in silico tool for predicting resistance to the antibiotics tested. This tool was then used to assess the allelic diversity of the penicillin-binding protein genes, which acquire mutations leading to beta-lactam antibiotic resistance, and found that they were highly conserved (≥80% shared) between the two populations. These findings show the potential of non-invasive isolates to serve as reservoirs of AMR determinants. | en_US |
dc.language.iso | eng | - |
dc.subject | Streptococcus pneumoniae | en_US |
dc.subject | antibiotic resistance | en_US |
dc.subject | genomics | en_US |
dc.title | Comparison of contemporary invasive and non-invasive Streptococcus pneumoniae isolates reveals new insights into circulating anti-microbial resistance determinants. | en_US |
dc.type | Journal Article | en_US |
dc.identifier.journaltitle | Antimicrobial Agents and Chemotherapy | en_US |
dc.identifier.affiliation | Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity , Melbourne, Victoria, Australia. | en_US |
dc.identifier.affiliation | Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity , Melbourne, Victoria, Australia. | en_US |
dc.identifier.affiliation | Communicable Diseases Branch, Department of Health , Victoria, Australia. | en_US |
dc.identifier.affiliation | Department of Microbiology, Monash Health , Clayton, Victoria, Australia. | en_US |
dc.identifier.affiliation | Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity , Melbourne, Victoria, Australia. | en_US |
dc.identifier.affiliation | Infectious Diseases | en_US |
dc.identifier.affiliation | Centre for Pathogen Genomics, University of Melbourne , Melbourne, Victoria, Australia. | en_US |
dc.identifier.affiliation | Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity , Melbourne, Victoria, Australia.;Department of Infectious Diseases, Austin Health , Heidelberg, Victoria, Australia.;Centre for Pathogen Genomics, University of Melbourne , Melbourne, Victoria, Australia. | en_US |
dc.identifier.affiliation | Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity , Melbourne, Victoria, Australia.;Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity , Melbourne, Victoria, Australia.;Centre for Pathogen Genomics, University of Melbourne , Melbourne, Victoria, Australia. | en_US |
dc.identifier.doi | 10.1128/aac.00785-23 | en_US |
dc.type.content | Text | en_US |
dc.identifier.orcid | 0000-0001-7364-7380 | en_US |
dc.identifier.orcid | 0000-0002-6155-8353 | en_US |
dc.identifier.orcid | 0000-0003-1596-4841 | en_US |
dc.identifier.orcid | 0000-0003-0150-123X | en_US |
dc.identifier.orcid | 0000-0003-0237-1473 | en_US |
dc.identifier.pubmedid | 37823632 | - |
dc.description.startpage | e0078523 | - |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | Journal Article | - |
crisitem.author.dept | Infectious Diseases | - |
crisitem.author.dept | Infectious Diseases | - |
crisitem.author.dept | Microbiology | - |
Appears in Collections: | Journal articles |
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