Please use this identifier to cite or link to this item:
https://ahro.austin.org.au/austinjspui/handle/1/32276
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Huber, Anne | - |
dc.contributor.author | Dijkstra, Christine | - |
dc.contributor.author | Ernst, Matthias | - |
dc.contributor.author | Eissmann, Moritz F | - |
dc.date | 2023 | - |
dc.date.accessioned | 2023-03-08T01:06:43Z | - |
dc.date.available | 2023-03-08T01:06:43Z | - |
dc.date.issued | 2023-03-17 | - |
dc.identifier.citation | STAR Protocols 2023-03-17; 4(1) | en_US |
dc.identifier.issn | 2666-1667 | - |
dc.identifier.uri | https://ahro.austin.org.au/austinjspui/handle/1/32276 | - |
dc.description.abstract | Gene-of-interest knockout organoids present a powerful and versatile research tool to study a gene's effects on many biological and pathological processes. Here, we present a straightforward and broadly applicable protocol to generate gene knockouts in mouse organoids using CRISPR-Cas9 technology. We describe the processes of transient transfecting organoids with pre-assembled CRISPR-Cas9 ribonucleoprotein complexes, organoid cell sorting, and establishing clonal organoid culture pairs. We then detail how to confirm the knockout via Western blot analysis. | en_US |
dc.language.iso | eng | - |
dc.subject | CRISPR | en_US |
dc.subject | Cancer | en_US |
dc.subject | Organoids | en_US |
dc.title | Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9. | en_US |
dc.type | Journal Article | en_US |
dc.identifier.journaltitle | STAR Protocols | en_US |
dc.identifier.affiliation | Olivia Newton-John Cancer Research Institute | en_US |
dc.identifier.affiliation | La Trobe School of Cancer Medicine, La Trobe University, Melbourne, VIC 3084, Australia. | en_US |
dc.identifier.doi | 10.1016/j.xpro.2023.102076 | en_US |
dc.type.content | Text | en_US |
dc.identifier.pubmedid | 36853714 | - |
dc.description.volume | 4 | - |
dc.description.issue | 1 | - |
dc.description.startpage | 102076 | - |
local.name.researcher | Dijkstra, Christine | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.openairetype | Journal Article | - |
item.cerifentitytype | Publications | - |
item.fulltext | No Fulltext | - |
crisitem.author.dept | Olivia Newton-John Cancer Research Institute | - |
crisitem.author.dept | Olivia Newton-John Cancer Research Institute | - |
crisitem.author.dept | Olivia Newton-John Cancer Research Institute | - |
Appears in Collections: | Journal articles |
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