Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/32276
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dc.contributor.authorHuber, Anne-
dc.contributor.authorDijkstra, Christine-
dc.contributor.authorErnst, Matthias-
dc.contributor.authorEissmann, Moritz F-
dc.date2023-
dc.date.accessioned2023-03-08T01:06:43Z-
dc.date.available2023-03-08T01:06:43Z-
dc.date.issued2023-03-17-
dc.identifier.citationSTAR Protocols 2023-03-17; 4(1)en_US
dc.identifier.issn2666-1667-
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/32276-
dc.description.abstractGene-of-interest knockout organoids present a powerful and versatile research tool to study a gene's effects on many biological and pathological processes. Here, we present a straightforward and broadly applicable protocol to generate gene knockouts in mouse organoids using CRISPR-Cas9 technology. We describe the processes of transient transfecting organoids with pre-assembled CRISPR-Cas9 ribonucleoprotein complexes, organoid cell sorting, and establishing clonal organoid culture pairs. We then detail how to confirm the knockout via Western blot analysis.en_US
dc.language.isoeng-
dc.subjectCRISPRen_US
dc.subjectCanceren_US
dc.subjectOrganoidsen_US
dc.titleGeneration of gene-of-interest knockouts in murine organoids using CRISPR-Cas9.en_US
dc.typeJournal Articleen_US
dc.identifier.journaltitleSTAR Protocolsen_US
dc.identifier.affiliationOlivia Newton-John Cancer Research Instituteen_US
dc.identifier.affiliationLa Trobe School of Cancer Medicine, La Trobe University, Melbourne, VIC 3084, Australia.en_US
dc.identifier.doi10.1016/j.xpro.2023.102076en_US
dc.type.contentTexten_US
dc.identifier.pubmedid36853714-
dc.description.volume4-
dc.description.issue1-
dc.description.startpage102076-
local.name.researcherDijkstra, Christine-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
crisitem.author.deptOlivia Newton-John Cancer Research Institute-
crisitem.author.deptOlivia Newton-John Cancer Research Institute-
crisitem.author.deptOlivia Newton-John Cancer Research Institute-
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