Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/27790
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dc.contributor.authorGuerra, Glen R-
dc.contributor.authorKong, Joseph C-
dc.contributor.authorMillen, Rosemary M-
dc.contributor.authorRead, Matthew-
dc.contributor.authorLiu, David Shi Hao-
dc.contributor.authorRoth, Sara-
dc.contributor.authorSampurno, Shienny-
dc.contributor.authorSia, Joseph-
dc.contributor.authorBernardi, Maria-Pia-
dc.contributor.authorChittleborough, Timothy J-
dc.contributor.authorBehrenbruch, Corina C-
dc.contributor.authorTeh, Jiasian-
dc.contributor.authorXu, Huiling-
dc.contributor.authorHaynes, Nicole M-
dc.contributor.authorYu, Jiaan-
dc.contributor.authorLupat, Richard-
dc.contributor.authorHawkes, David-
dc.contributor.authorDi Costanzo, Natasha-
dc.contributor.authorTothill, Richard W-
dc.contributor.authorMitchell, Catherine-
dc.contributor.authorNgan, Samuel Y-
dc.contributor.authorHeriot, Alexander G-
dc.contributor.authorRamsay, Robert G-
dc.contributor.authorPhillips, Wayne A-
dc.date2021-10-18-
dc.date.accessioned2021-10-25T22:33:40Z-
dc.date.available2021-10-25T22:33:40Z-
dc.date.issued2021-10-18-
dc.identifier.citationCell Death & Disease 2021; 12(11): 959en
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/27790-
dc.description.abstractAnal cancer is a rare disease that has doubled in incidence over the last four decades. Current treatment and survival of patients with this disease has not changed substantially over this period of time, due, in part, to a paucity of preclinical models to assess new therapeutic options. To address this hiatus, we set-out to establish, validate and characterise a panel of human anal squamous cell carcinoma (ASCC) cell lines by employing an explant technique using fresh human ASCC tumour tissue. The panel of five human ASCC cell lines were validated to confirm their origin, squamous features and tumourigenicity, followed by molecular and genomic (whole-exome sequencing) characterisation. This panel recapitulates the genetic and molecular characteristics previously described in ASCC including phosphoinositide-3-kinase (PI3K) mutations in three of the human papillomavirus (HPV) positive lines and TP53 mutations in the HPV negative line. The cell lines demonstrate the ability to form tumouroids and retain their tumourigenic potential upon xenotransplantation, with varied inducible expression of major histocompatibility complex class I (MHC class I) and Programmed cell death ligand 1 (PD-L1). We observed differential responses to standard chemotherapy, radiotherapy and a PI3K specific molecular targeted agent in vitro, which correlated with the clinical response of the patient tumours from which they were derived. We anticipate this novel panel of human ASCC cell lines will form a valuable resource for future studies into the biology and therapeutics of this rare disease.en
dc.language.isoeng-
dc.titleMolecular and genomic characterisation of a panel of human anal cancer cell lines.en
dc.typeJournal Articleen
dc.identifier.journaltitleCell Death & Diseaseen
dc.identifier.affiliationCentre for Cancer Research, The University of Melbourne, Parkville, VIC, 3010, Australiaen
dc.identifier.affiliationDivision of Radiation Oncology, Peter MacCallum Cancer Centre, Melbourne, VIC, 3000, Australiaen
dc.identifier.affiliationDepartment of Pathology, Peter MacCallum Cancer Centre, Melbourne, VIC, 3000, Australiaen
dc.identifier.affiliationDepartment of Biochemistry and Pharmacology, The University of Melbourne, Parkville, VIC, 3010, Australiaen
dc.identifier.affiliationVCS Foundation, Carlton, VIC, 3053, Australiaen
dc.identifier.affiliationDepartment of Pathology, University of Malaya, Kuala Lumpur, Malaysiaen
dc.identifier.affiliationDepartment of Surgery, St Vincent's Hospital, The University of Melbourne, Parkville, VIC, 3010, Australiaen
dc.identifier.affiliationDivision of Cancer Research, Peter MacCallum Cancer Centre, Melbourne, VIC, 3000, Australiaen
dc.identifier.affiliationDivision of Cancer Surgery, Peter MacCallum Cancer Centre, Melbourne, VIC, 3000, Australiaen
dc.identifier.affiliationThe Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, VIC, 3010, Australiaen
dc.identifier.affiliationDepartment of Clinical Pathology, The University of Melbourne, Parkville, VIC, 3010, Australiaen
dc.identifier.affiliationSurgeryen
dc.identifier.doi10.1038/s41419-021-04141-5en
dc.type.contentTexten
dc.identifier.orcid0000-0002-2505-1643en
dc.identifier.orcid0000-0001-5003-0433en
dc.identifier.orcid0000-0002-7961-638Xen
dc.identifier.pubmedid34663790-
local.name.researcherLiu, David Shi Hao
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
crisitem.author.deptSurgery-
crisitem.author.deptSurgery (University of Melbourne)-
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