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DC Field | Value | Language |
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dc.contributor.author | Tipping, P G | - |
dc.contributor.author | Buchanan, Russell R C | - |
dc.contributor.author | Riglar, A G | - |
dc.contributor.author | Dimech, W J | - |
dc.contributor.author | Littlejohn, G O | - |
dc.contributor.author | Holdsworth, S R | - |
dc.date.accessioned | 2021-10-07T23:08:14Z | - |
dc.date.available | 2021-10-07T23:08:14Z | - |
dc.date.issued | 1991-01 | - |
dc.identifier.citation | Pathology 1991; 23(1): 21-4 | en |
dc.identifier.issn | 0031-3025 | |
dc.identifier.uri | https://ahro.austin.org.au/austinjspui/handle/1/27683 | - |
dc.description.abstract | One hundred and twenty six sera from 116 patients with systemic lupus erythematosus (SLE) and from 51 control patients were assayed for the presence of anti-DNA antibodies, using a commercial enzyme linked immunosorbent assay (ELISA). Fifty three sera (42%) from SLE patients were positive and a further 13 sera (10%) fell in the 'equivocal' positive range. Three control sera were positive. In a standard 14C DNA Farr assay, 67 sera (53%) from SLE patients were positive. One control serum was weakly positive. There was a good linear correlation between absorption in the ELISA and the 14C DNA binding result (r = 0.73). Results in the ELISA and Farr assays were concordant in 96 of the 126 SLE sera, and 47 of 51 control sera. Sequential sera from a further 6 patients with fluctuating clinical activity of SLE showed similar patterns of change of anti-DNA antibodies in both assays. The ELISA was more sensitive than the Crithidia luciliae immunofluorescence assay which detected 44 positive sera (35%) in the SLE group. These results suggest that this ELISA assay may be a useful alternative to the Crithidia assay or an effective screen prior to testing in the more technically difficult and time consuming Farr assay for the measurement of anti-DNA antibodies. | en |
dc.language.iso | eng | |
dc.title | Measurement of anti-DNA antibodies by ELISA: a comparative study with Crithidia and a Farr assay. | en |
dc.type | Journal Article | en |
dc.identifier.journaltitle | Pathology | en |
dc.identifier.affiliation | Department of Medicine, Monash University, Prince Henry's Hospital, Melbourne | en |
dc.identifier.affiliation | Medicine (University of Melbourne) | en |
dc.identifier.doi | 10.3109/00313029109061435 | en |
dc.type.content | Text | en |
dc.identifier.pubmedid | 2062564 | |
local.name.researcher | Buchanan, Russell R C | |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
item.openairetype | Journal Article | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Rheumatology | - |
Appears in Collections: | Journal articles |
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