Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/27683
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dc.contributor.authorTipping, P G-
dc.contributor.authorBuchanan, Russell R C-
dc.contributor.authorRiglar, A G-
dc.contributor.authorDimech, W J-
dc.contributor.authorLittlejohn, G O-
dc.contributor.authorHoldsworth, S R-
dc.date.accessioned2021-10-07T23:08:14Z-
dc.date.available2021-10-07T23:08:14Z-
dc.date.issued1991-01-
dc.identifier.citationPathology 1991; 23(1): 21-4en
dc.identifier.issn0031-3025
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/27683-
dc.description.abstractOne hundred and twenty six sera from 116 patients with systemic lupus erythematosus (SLE) and from 51 control patients were assayed for the presence of anti-DNA antibodies, using a commercial enzyme linked immunosorbent assay (ELISA). Fifty three sera (42%) from SLE patients were positive and a further 13 sera (10%) fell in the 'equivocal' positive range. Three control sera were positive. In a standard 14C DNA Farr assay, 67 sera (53%) from SLE patients were positive. One control serum was weakly positive. There was a good linear correlation between absorption in the ELISA and the 14C DNA binding result (r = 0.73). Results in the ELISA and Farr assays were concordant in 96 of the 126 SLE sera, and 47 of 51 control sera. Sequential sera from a further 6 patients with fluctuating clinical activity of SLE showed similar patterns of change of anti-DNA antibodies in both assays. The ELISA was more sensitive than the Crithidia luciliae immunofluorescence assay which detected 44 positive sera (35%) in the SLE group. These results suggest that this ELISA assay may be a useful alternative to the Crithidia assay or an effective screen prior to testing in the more technically difficult and time consuming Farr assay for the measurement of anti-DNA antibodies.en
dc.language.isoeng
dc.titleMeasurement of anti-DNA antibodies by ELISA: a comparative study with Crithidia and a Farr assay.en
dc.typeJournal Articleen
dc.identifier.journaltitlePathologyen
dc.identifier.affiliationDepartment of Medicine, Monash University, Prince Henry's Hospital, Melbourneen
dc.identifier.affiliationMedicine (University of Melbourne)en
dc.identifier.doi10.3109/00313029109061435en
dc.type.contentTexten
dc.identifier.pubmedid2062564
local.name.researcherBuchanan, Russell R C
item.fulltextNo Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
crisitem.author.deptRheumatology-
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