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dc.contributor.authorCheung, Ernest-
dc.contributor.authorNikfarjam, Mehrdad-
dc.contributor.authorJackett, Louise A-
dc.contributor.authorBolton, Damien M-
dc.contributor.authorIschia, Joseph J-
dc.contributor.authorPatel, Oneel-
dc.identifier.citationJournal of clinical and experimental hepatology 2020; 10(3): 228-235-
dc.description.abstractIschaemia-reperfusion injury (IRI) is a major obstacle during liver transplantation and resection surgeries for cancer, with a need for effective and safe drugs to reduce IRI. Zinc preconditioning has been shown to protect against liver IRI in a partial (70%) ischaemia model. However, its efficacy against a clinically relevant Pringle manoeuvre that results in global liver ischaemia (100%) is unknown. The aim of this study was to test the efficacy of zinc preconditioning in a rat model of global liver ischaemia. Rats were preconditioned via subcutaneous injection of 10 mg/kg of ZnCl2, 24 h and 4 h before ischaemia. Total liver ischaemia (100%) was induced by placing a clamp across the portal triad for 30 min. Liver injury was assessed by serum alanine transaminase (ALT) and aspartate transaminase (AST) levels in blood taken before ischaemia (baseline) and at 1, 2, 4, 24, 48, 72, 96 and 120 hours after ischaemia. Animals were culled after 7 days, and the harvested livers were histologically analysed. On a two-way repeated-measures analysis of variance, there was a statistically significant (p = 0.025) difference in the mean ALT levels between saline- and ZnCl2-treated groups. Specifically at 24 h after ischaemia, the ALT (341 ± 99 U/L) and AST (606 ± 78 U/L) in the zinc-treated group were significantly less than the ALT (2863 ± 828 U/L) and AST (3591 ± 948 U/L) values in the saline-treated group. Zinc significantly reduced neutrophil infiltration and necrosis compared with the saline control. Zinc preconditioning reduces the overall hepatocellular damage from IRI. These results lay the foundation to assess the benefit of zinc preconditioning for clinical applications.-
dc.subjectALT, Alanine Transaminase-
dc.subjectANOVA, Analysis of Variance-
dc.subjectAST, Aspartate Transaminase-
dc.subjectIRI, Ischaemia-Reperfusion Injury-
dc.titleThe Protective Effect of Zinc Against Liver Ischaemia Reperfusion Injury in a Rat Model of Global Ischaemia.-
dc.typeJournal Article-
dc.identifier.journaltitleJournal of clinical and experimental hepatology-
dc.identifier.affiliationDepartment of Surgery, The University of Melbourne, Victoria, Australiaen
dc.identifier.affiliationDepartment of Anatomical Pathology, Austin Health, Heidelberg, Victoria, Australiaen
dc.identifier.affiliationDepartment of Urology, Austin Health, Heidelberg, Victoria, Australiaen
dc.type.austinJournal Article-
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