Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/19377
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dc.contributor.authorSenthilkumaran, M-
dc.contributor.authorBobrovskaya, L-
dc.contributor.authorVerberne, Anthony J M-
dc.contributor.authorLlewellyn-Smith, Ida J-
dc.date2018-08-23-
dc.date.accessioned2018-09-17T01:47:03Z-
dc.date.available2018-09-17T01:47:03Z-
dc.date.issued2018-
dc.identifier.citationThe Journal of comparative neurology 2018; 526(16): 2665-2682-
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/19377-
dc.description.abstractLow blood glucose activates brainstem adrenergic and cholinergic neurons, driving adrenaline secretion from the adrenal medulla and glucagon release from the pancreas. Despite their roles in maintaining glucose homeostasis, the distributions of insulin-responsive adrenergic and cholinergic neurons in the medulla are unknown. We fasted rats overnight and gave them insulin (10 IU/kg i.p.) or saline after 2 weeks of handling. Blood samples were collected before injection and before perfusion at 90 min. We immunoperoxidase-stained transverse sections of perfused medulla to show Fos plus either phenylethanolamine N-methyltransferase (PNMT) or choline acetyltransferase (ChAT). Insulin injection lowered blood glucose from 4.9+0.3 mmol/L to 1.7+0.2 mmol/L (mean+SEM; n=6); saline injection had no effect. In insulin-treated rats, many PNMT-immunoreactive C1 neurons had Fos-immunoreactive nuclei, with the proportion of activated neurons being highest in the caudal part of the C1 column. In the rostral ventrolateral medulla, 33.3%±1.4% (n=8) of C1 neurons were Fos-positive. Insulin also induced Fos in 47.2%±2.0% (n=5) of dorsal medullary C3 neurons and in some C2 neurons. In the dorsal motor nucleus of the vagus (DMV), insulin evoked Fos in many ChAT-positive neurons. Activated neurons were concentrated in the medial and middle regions of the DMV beneath and just rostral to the area postrema. In control rats, very few C1, C2 or C3 neurons and no DMV neurons were Fos-positive. The high numbers of PNMT-immunoreactive and ChAT-immunoreactive neurons that express Fos after insulin treatment reinforce the importance of these neurons in the central response to a decrease in glucose bioavailability. This article is protected by copyright. All rights reserved.-
dc.language.isoeng-
dc.subjectBlood glucose-
dc.subjectC1 neurons-
dc.subjectC3 neurons-
dc.subjectFos-
dc.subjectRRID:AB_2079751-
dc.subjectRRID:AB_2231996-
dc.subjectRRID:AB_2340397-
dc.subjectRRID:AB_2340593-
dc.subjectRRID:AB_262016-
dc.subjectRVLM-
dc.subjectdorsal vagal motor neurons-
dc.titleInsulin-responsive autonomic neurons in rat medulla oblongata-
dc.typeJournal Article-
dc.identifier.journaltitleThe Journal of comparative neurology-
dc.identifier.affiliationCardiovascular Medicine, Human Physiology and Centre for Neuroscience, College of Medicine and Public Health, Flinders University, Bedford Park, SA, Australiaen
dc.identifier.affiliationClinical Pharmacology and Therapeutics Unit, Department of Medicine, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australiaen
dc.identifier.affiliationSchool of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA, Australiaen
dc.identifier.doi10.1002/cne.24523-
dc.identifier.orcid0000-0003-4269-6846en
dc.identifier.orcid0000-0002-2049-1439en
dc.identifier.orcid0000-0002-8748-3660en
dc.identifier.orcid0000-0002-5793-6147en
dc.identifier.pubmedid30136719-
dc.type.austinJournal Article-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.openairetypeJournal Article-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
crisitem.author.deptClinical Pharmacology and Therapeutics-
crisitem.author.deptMedicine (University of Melbourne)-
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